ReviewMethods (San Diego, Calif.)2017
Imaging flow cytometry analysis of intracellular pathogens.
Review in Methods (San Diego, Calif.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 68 citations in OpenAlex.
- Review
- Intercellular Mitochondrial Transfer: Implications in Cardiovascular Health.Circulation research · 2026Review
- Article
- Highly Efficient Calibration-Free Color Compensation Algorithm for Imaging Flow Cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2025Article
- Imaging flow cytometry as a novel approach for the diagnosis of heparin-induced thrombocytopenia.British journal of haematology · 2025Article
- Imaging flow cytometry reveals the mechanism of equine arteritis virus entry and internalization.Scientific reports · 2025Article
- Elucidating siRNA Cellular Delivery Mechanism Mediated by Quaternized Starch Nanoparticles.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Mutual Inhibition of Antithrombin III and SARS-CoV-2 Cellular Attachment to Syndecans: Implications for COVID-19 Treatment and Vaccination.International journal of molecular sciences · 2024Article
- Imaging Flow Cytometry: Development, Present Applications, and Future Challenges.Methods and protocols · 2024Review
- Article
- Intravital Microscopy for Imaging and Live Cell Tracking of Alveolar Macrophages in Real Time.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Syndecan-4 Mediates the Cellular Entry of Adeno-Associated Virus 9.International journal of molecular sciences · 2023Article
- Opportunities in optical and electrical single-cell technologies to study microbial ecosystems.Frontiers in microbiology · 2023Review
- TLR4/CD14/MD2 Revealed as the Limited Toll-like Receptor Complex forMicroorganisms · 2022Article
- Syndecan-4 Is a Key Facilitator of the SARS-CoV-2 Delta Variant's Superior Transmission.International journal of molecular sciences · 2022Article
- Paving the Way: Contributions of Big Data to Apicomplexan and Kinetoplastid Research.Frontiers in cellular and infection microbiology · 2022Review
- Novel dual-fluorescent flow cytometric approach for quantification of macrophages infected withParasitology · 2022Article
- Contribution of Syndecans to the Cellular Entry of SARS-CoV-2.International journal of molecular sciences · 2021Article
- Eukaryotic Cell Capture by Amplified MagneticFrontiers in microbiology · 2021Article
- The Application of Imaging Flow Cytometry for Characterisation and Quantification of Bacterial Phenotypes.Frontiers in cellular and infection microbiology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
Imaging flow cytometry has been applied to address questions in infection biology, in particular, infections induced by intracellular pathogens. This methodology, which utilizes specialized analytic software makes it possible to analyze hundreds of quantified features for hundreds of thousands of individual cellular or subcellular events in a single experiment. Imaging flow cytometry analysis of host cell-pathogen interaction can thus quantitatively addresses a variety of biological questions related to intracellular infection, including cell counting, internalization score, and subcellular patterns of co-localization. Here, we provide an overview of recent achievements in the use of fluorescently labeled prokaryotic or eukaryotic pathogens in human cellular infections in analysis of host-pathogen interactions. Specifically, we give examples of Imagestream-based analysis of cell lines infected with Toxoplasma gondii or Mycobacterium tuberculosis. Furthermore, we illustrate the capabilities of imaging flow cytometry using a combination of standard IDEAS™ software and the more recently developed Feature Finder algorithm, which is capable of identifying statistically significant differences between researcher-defined image galleries. We argue that the combination of imaging flow cytometry with these software platforms provides a powerful new approach to understanding host control of intracellular pathogens.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.