ArticleNature communications2016
N-glycosylation enables high lateral mobility of GPI-anchored proteins at a molecular crowding threshold.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 40 citations in OpenAlex.
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- Sub-membrane actin rings compartmentalize the plasma membrane.The Journal of cell biology · 2024Article
- X-ray reflectivity study of the heat shock protein Hsp70 interaction with an artificial cell membrane model.Scientific reports · 2023Article
- Common and unique features of glycosylation and glycosyltransferases in African trypanosomes.The Biochemical journal · 2022Article
- Structure of trypanosome coat protein VSGsur and function in suramin resistance.Nature microbiology · 2021Article
- Mutations Suppressing the Lack of Prepilin Peptidase Provide Insights Into the Maturation of the Major Pilin Protein in Cyanobacteria.Frontiers in microbiology · 2021Article
- To the Surface and Back: Exo- and Endocytic Pathways inFrontiers in cell and developmental biology · 2021Review
- The Glycosylphosphatidylinositol Anchor: A Linchpin for Cell Surface Versatility of Trypanosomatids.Frontiers in cell and developmental biology · 2021Review
- TheiScience · 2020Article
- Multiparametric Atomic Force Microscopy Identifies Multiple Structural and Physical Heterogeneities on the Surface ofACS omega · 2020Article
- African trypanosomes expressing multiple VSGs are rapidly eliminated by the host immune system.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Structural basis for the shielding function of the dynamic trypanosome variant surface glycoprotein coat.Nature microbiology · 2017Article
- Facilitating trypanosome imaging.Experimental parasitology · 2017Article
- A Receptor's Tale: An Eon in the Life of a Trypanosome Receptor.PLoS pathogens · 2017Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The protein density in biological membranes can be extraordinarily high, but the impact of molecular crowding on the diffusion of membrane proteins has not been studied systematically in a natural system. The diversity of the membrane proteome of most cells may preclude systematic studies. African trypanosomes, however, feature a uniform surface coat that is dominated by a single type of variant surface glycoprotein (VSG). Here we study the density-dependence of the diffusion of different glycosylphosphatidylinositol-anchored VSG-types on living cells and in artificial membranes. Our results suggest that a specific molecular crowding threshold (MCT) limits diffusion and hence affects protein function. Obstacles in the form of heterologous proteins compromise the diffusion coefficient and the MCT. The trypanosome VSG-coat operates very close to its MCT. Importantly, our experiments show that N-linked glycans act as molecular insulators that reduce retarding intermolecular interactions allowing membrane proteins to function correctly even when densely packed.
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