ArticleScientific reports2016
Papain gel containing methylene blue for simultaneous caries removal and antimicrobial photoinactivation against Streptococcus mutans biofilms.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Antibacterial and Photocatalytic Performance of EPS-Stabilized Silver Nanoparticles Immobilized on Activated Natural Zeolite.Polymers · 2026Article
- Photodynamic therapy for caries management: mechanisms, applications, and perspectives.Frontiers in cellular and infection microbiology · 2026Review
- Histological Evaluation of Chemo Mechanical Caries Removal with a Babaco-Based Formulation Gel.Gels (Basel, Switzerland) · 2025Article
- Antimicrobial Efficacy of Chemomechanical Carie Removal Agents-A Systematic Integrative Review.Biomedicines · 2024Review
- New Technological Approaches for Dental Caries Treatment: From Liquid Crystalline Systems to Nanocarriers.Pharmaceutics · 2023Review
- Chemonucleolysis combined with dynamic loading for inducing degeneration in bovine caudal intervertebral discs.Frontiers in bioengineering and biotechnology · 2023Article
- Article
- The Contribution of Photodynamic Inactivation vs. Corsodyl Mouthwash to the Control of Streptococcus mutans Biofilms.Current microbiology · 2020Article
- One-step Synthesized Silver Nanoparticles Using Isoimperatorin: Evaluation of Photocatalytic, and Electrochemical Activities.Scientific reports · 2020Article
- Article
- Antimicrobial photodynamic therapy on teeth with molar incisor hypomineralization-controlled clinical trial.Medicine · 2019Article
- Antibacterial Activities of Herbal Toothpastes Combined with Essential Oils againstPathogens (Basel, Switzerland) · 2019Article
- Assessment of Chitosan-Based Hydrogel and Photodynamic Inactivation against Propionibacterium acnes.Molecules (Basel, Switzerland) · 2018Article
- Study of Methylene Blue Ototoxicity in the Guinea Pig.Journal of clinical medicine research · 2017Article
- Article
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
This study intended to evaluate the effects of a papain-gel with a red-light absorbing pigment (methylene blue - MB) to mediate photodynamic therapy (PDT) against Streptococcus mutans biofilms. The PapaMBlue was compared with free MB to generate reactive oxygen species using fluorescence probes (SOSG and HPF). PDT (660-nm light) was carried out against S. mutans biofilms grown on either plastic dishes or on collagen membrane and assayed by CFU, live-dead staining using confocal microscopy, transmission electron microscopy and H&E staining for collagen films. Cytotoxicity and subcellular localization was studied in human fibroblasts. Sponges of bioabsorbable type I collagen membrane were exposed to papain based gel, irradiated with laser and analyzed about their integrity by ATR-FTIR. The PapaMBlue produced higher amounts of singlet oxygen and hydroxyl radicals than free MB, possibly due to better disaggregation of the dye in solution. The PapaMBlue antimicrobial effects on biofilms proved to be capable of reducing the S. mutans. Both MTT and PrestoBlue assays showed higher cell viability and metabolism scores in fibroblasts treated with PapaMBlue and MB, possibly due to stimulation of mitochondrial activity and that collagen triple helix is unaffected. The PapaMBlue is equally effective as MB in destroying S. mutans biofilms growing on plastic or collagen without affecting fibroblasts.
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Registered trials
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