Evidence map›Paper›PMID 27641099›Full record

ArticleCell metabolism2016

AMPK/α-Ketoglutarate Axis Dynamically Mediates DNA Demethylation in the Prdm16 Promoter and Brown Adipogenesis.

Qiyuan Yang, Xingwei Liang, Xiaofei Sun, Lupei Zhang, Xing Fu, Carl J Rogers, Anna Berim, Shuming Zhang, Songbo Wang, Bo Wang and 6 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell metabolism, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 149 papers.

0numbers the graph read from it
0cells of the map it votes in
149citing papers in PubMed
13.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

149 citing papers in PubMed, 232 citations in OpenAlex.

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  17. Hederagenin fromPlants (Basel, Switzerland) · 2024
    Article
  18. Journal of microbiology and biotechnology · 2024
    Article
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89 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 3 countries.

Qiyuan YangWashington Center for Muscle Biology and Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA.
Xingwei LiangWashington Center for Muscle Biology and Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA.
Xiaofei SunSchool of Food Sciences, Washington State University, Pullman, WA 99164, USA.
Lupei ZhangWashington Center for Muscle Biology and Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Xing FuWashington Center for Muscle Biology and Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA.
Carl J RogersWashington Center for Muscle Biology and Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA.
Anna BerimInstitute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA.
Shuming ZhangSchool of Food Sciences, Washington State University, Pullman, WA 99164, USA.
Songbo WangWashington Center for Muscle Biology and Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA.
Bo WangWashington Center for Muscle Biology and Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA.
Marc ForetzINSERM U1016, Institut Cochin, 75014 Paris, France; CNRS UMR 8104, 75014 Paris, France; Université Paris Descartes, Sorbonne Paris Cité, 75006 Paris, France.
Benoit ViolletINSERM U1016, Institut Cochin, 75014 Paris, France; CNRS UMR 8104, 75014 Paris, France; Université Paris Descartes, Sorbonne Paris Cité, 75006 Paris, France.
David R GangInstitute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA.
Buel D RodgersWashington Center for Muscle Biology and Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA.
Mei-Jun ZhuSchool of Food Sciences, Washington State University, Pullman, WA 99164, USA.
Min DuWashington Center for Muscle Biology and Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA; Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100194, China. Electronic address: min.du@wsu.edu.
Washington State University · USWashington Center · USCentre National de la Recherche Scientifique · FRSorbonne Paris Cité · FR

Funding

Maternal obesity, AMPK and fetal brown adipogenesisR01HD067449 · NICHD · WASHINGTON STATE UNIVERSITY · PI MIN DU · 2010 to 2026
$4.6M
Zfp423 and Progenitor Adipogenesis during AgingR21AG049976 · NIA · WASHINGTON STATE UNIVERSITY · PI DU, MIN · 2016 to 2017
$405k
NIA NIH HHS R21 AG049976NICHD NIH HHS R01 HD067449
6 · The paper itself

Abstract

Promoting brown adipose tissue (BAT) development is an attractive strategy for the treatment of obesity, as activated BAT dissipates energy through thermogenesis; however, the mechanisms controlling BAT formation are not fully understood. We hypothesized that as a master regulator of energy metabolism, AMP-activated protein kinase (AMPK) may play a direct role in the process and found that AMPKα1 (PRKAA1) ablation reduced Prdm16 expression and impaired BAT development. During early brown adipogenesis, the cellular levels of α-ketoglutarate (αKG), a key metabolite required for TET-mediated DNA demethylation, were profoundly increased and required for active DNA demethylation of the Prdm16 promoter. AMPKα1 ablation reduced isocitrate dehydrogenase 2 activity and cellular αKG levels. Remarkably, postnatal AMPK activation with AICAR or metformin rescued obesity-induced suppression of brown adipogenesis and thermogenesis. In summary, AMPK is essential for the epigenetic control of BAT development through αKG, thus linking a metabolite to progenitor cell differentiation and thermogenesis.

Indexed as

Promoter Regions, GeneticAdipogenesisAdipose Tissue, BrownAMP-Activated Protein KinasesAnimalsCell LineDNA-Binding ProteinsDNA MethylationGene DeletionKetoglutaric AcidsMice, KnockoutMice, ObeseObesityStromal CellsTranscription FactorsWeaningAMP-Activated Protein KinasesDNA-Binding ProteinsKetoglutaric AcidsPrdm16 protein, mouseTranscription Factorsbrown adipogenesisDNA demethylationPrkaa1

Identifiers

PMID27641099
PMCPMC5061633
OpenAlexW2519117803

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.