ArticleJournal of oral microbiology2016
Actinomyces spp. gene expression in root caries lesions.
Article in Journal of oral microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- Host-microbiome-immune disequilibrium in oral disease: mechanisms, dysbiosis, and precision therapeutics.Frontiers in immunology · 2026Review
- Shotgun metagenomic profiling reveals ecological and functional alterations of the oral microbiome in craniosynostosis.Journal of oral microbiology · 2026Article
- Oral dysbiosis: methodological evolution, the mobile resistome and the future of machine learning in dentistry.Journal of oral microbiology · 2026Review
- Bacteriophage-mediated biofilm control: a novel targeted strategy for the management of dental caries.Frontiers in cellular and infection microbiology · 2026Review
- Diabetes alters the supragingival microbiome through plasma-to-saliva migration of glucose and fructose.Microbiome · 2025Article
- Effect of cold atmospheric plasma on common oral pathogenic microorganisms: a narrative review.Annals of medicine · 2025Review
- Evaluating microbial regulation as a preventive strategy for radiation-related caries: A review.Head & face medicine · 2025Review
- Oral Microbiome Analysis and Caries Risk Classification Using the Caries Management by Risk Assessment System in Pre-Orthodontic Patients.Journal of clinical medicine · 2025Article
- Article
- Development of a Coculture Model for Assessing Competing Host Mammalian Cell and Bacterial Attachment on Zirconia versus Titanium.ACS biomaterials science & engineering · 2024Article
- Oral microbial profiles of extrinsic black tooth stain in primary dentition: A literature review.Journal of dental sciences · 2024Review
- Exploring Salivary Metabolic Alterations in Type 2 Diabetes: Implications for Dental Caries and Potential Influences of HbA1c and Vitamin D Levels.Metabolites · 2024Article
- The Evolving Microbiome of Dental Caries.Microorganisms · 2024Review
- The action of microbial collagenases in dentinal matrix degradation in root caries and potential strategies for its management: a comprehensive state-of-the-art review.Journal of applied oral science : revista FOB · 2024Review
- Antibacterial Properties of the Antimicrobial Peptide Gallic Acid-Polyphemusin I (GAPI).Antibiotics (Basel, Switzerland) · 2023Article
- Digital imaging and qPCR analysis and comparison of short-term plaque removal effects of toothbrushing.Frontiers in dental medicine · 2023Article
- Functional changes in the oral microbiome after use of fluoride and arginine containing dentifrices: a metagenomic and metatranscriptomic study.Microbiome · 2022Article
- Antibacterial and antioxidant effect of ethanol extracts of Terminalia chebula on Streptococcus mutans.Clinical and experimental dental research · 2021Article
- Periodontal Pathogens Inhabit Root Caries Lesions Extending beyond the Gingival Margin: A Next-Generation Sequencing Analysis.Microorganisms · 2021Article
- Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundThe studies of the distribution of Actinomyces spp. on carious and non-carious root surfaces have not been able to confirm the association of these bacteria with root caries, although they were extensively implicated as a prime suspect in root caries.
objectiveThe aim of this study was to observe the gene expression of Actinomyces spp. in the microbiota of root surfaces with and without caries.
designThe oral biofilms from exposed sound root surface (SRS; n=10) and active root caries (RC; n=30) samples were collected. The total bacterial RNA was extracted, and the mRNA was isolated. Samples with low RNA concentration were pooled, yielding a final sample size of SRS=10 and RC=9. Complementary DNA (cDNA) libraries were prepared and sequenced on an Illumina(®) HiSeq 2500 system. Sequence reads were mapped to eight Actinomyces genomes. Count data were normalized using DESeq2 to analyse differential gene expression applying the Benjamini-Hochberg correction (false discovery rate [FDR]<0.001).
resultsActinomyces spp. had similar numbers of reads (Mann-Whitney U-test; p>0.05), except for Actinomyces OT178 (p=0.001) and Actinomyces gerencseriae (p=0.004), which had higher read counts in the SRS. Genes that code for stress proteins (clp, dnaK, and groEL), enzymes of glycolysis pathways (including enolase and phosphoenolpyruvate carboxykinase), adhesion (Type-2 fimbrial and collagen-binding protein), and cell growth (EF-Tu) were highly - but not differentially (p>0.001) - expressed in both groups. Genes with the most significant upregulation in RC were those coding for hypothetical proteins and uracil DNA glycosylase (p=2.61E-17). The gene with the most significant upregulation in SRS was a peptide ABC transporter substrate-binding protein (log2FC=-6.00, FDR=2.37E-05).
conclusionThere were similar levels of Actinomyces gene expression in both sound and carious root biofilms. These bacteria can be commensal in root surface sites but may be cariogenic due to survival mechanisms that allow them to exist in acid environments and to metabolize sugars, saving energy.
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Registered trials
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