ArticleJournal of virological methods2016
Generation and Characterization of Eptesicus fuscus (Big brown bat) kidney cell lines immortalized using the Myotis polyomavirus large T-antigen.
Article in Journal of virological methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 38 citations in OpenAlex.
- Protocol for collection and generation of bat primary lung and kidney cells.STAR protocols · 2026Article
- Chiropteran (Animals : an open access journal from MDPI · 2026Article
- Understanding Climate Change Threats to Vertebrate Wildlife by Studying Ecoimmunology Across Biological Scales.Integrative and comparative biology · 2025Review
- From Development to Regeneration: Insights into Flight Muscle Adaptations from Bat Muscle Cell Lines.Cells · 2025Article
- Convergent evolution in nucleocapsid facilitated SARS-CoV-2 adaptation for human infection.Journal of virology · 2025Article
- Bat-specific adaptations in interferon signaling and GBP1 contribute to enhanced antiviral capacity.Nature communications · 2025Article
- Establishment of a cell line from the hematophagous Bat Desmodus rotundus susceptible to Lyssavirus rabies.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Expanding the bat toolbox: Carollia perspicillata bat cell lines and reagents enable the characterization of viral susceptibility and innate immune responses.PLoS biology · 2025Article
- Studying bats using a One Health lens: bridging the gap between bat virology and disease ecology.Journal of virology · 2024Review
- Generating bat primary and immortalised cell-lines from wing biopsies.Scientific reports · 2024Article
- Tissue and cellular tropism ofVeterinary pathology · 2024Article
- Establishment of immortalized Egyptian Rousettus bat cell lines.FEBS open bio · 2024Article
- Bat behavioral immune responses in social contexts: current knowledge and future directions.Frontiers in immunology · 2023Review
- From bats to pangolins: new insights into species differences in the structure and function of the immune system.Innate immunity · 2022Review
- Coronavirus, the King Who Wanted More Than a Crown: From Common to the Highly Pathogenic SARS-CoV-2, Is the Key in the Accessory Genes?Frontiers in microbiology · 2021Review
- Novel and Diverse Non-Rabies Rhabdoviruses Identified in Bats with Human Exposure, South Dakota, USA.Viruses · 2020Article
- Animal Models to Study Emerging Technologies Against SARS-CoV-2.Cellular and molecular bioengineering · 2020Article
- Selection of viral variants during persistent infection of insectivorous bat cells with Middle East respiratory syndrome coronavirus.Scientific reports · 2020Article
- Novel Insights Into Immune Systems of Bats.Frontiers in immunology · 2020Review
- Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
It is speculated that bats are important reservoir hosts for numerous viruses, with 27 viral families reportedly detected in bats. Majority of these viruses have not been isolated and there is little information regarding their biology in bats. Establishing a well-characterized bat cell line supporting the replication of bat-borne viruses would facilitate the analysis of virus-host interactions in an in vitro model. Currently, few bat cell lines have been developed and only Tb1-Lu, derived from Tadarida brasiliensis is commercially available. Here we describe a method to establish and immortalize big brown bat (Eptesicus fuscus) kidney (Efk3) cells using the Myotis polyomavirus T-antigen. Subclones of this cell line expressed both epithelial and fibroblast markers to varying extents. Cell clones expressed interferon beta in response to poly(I:C) stimulation and supported the replication of four different viruses, namely, vesicular stomatitis virus (VSV), porcine epidemic diarrhea coronavirus (PED-CoV), Middle-East respiratory syndrome coronavirus (MERS-CoV) and herpes simplex virus (HSV). To our knowledge, this is the first bat cell line from a northern latitude insectivorous bat developed using a novel technology. The cell line has the potential to be used for isolation of bat viruses and for studying virus-bat interactions in culture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.