Evidence map›Paper›PMID 27638947›Full record

ArticleThe Journal of infectious diseases2016

Molecular Diagnostic Field Test for Point-of-Care Detection of Ebola Virus Directly From Blood.

Jason W Benzine, Kerry M Brown, Krystle N Agans, Ronald Godiska, Chad E Mire, Krishne Gowda, Brandon Converse, Thomas W Geisbert, David A Mead, Yogesh Chander

Open access · bronzeAbstract read
In one paragraph

Article in The Journal of infectious diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 33 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Improved protocol forExperimental biology and medicine (Maywood, N.J.) · 2023
    Article
  5. Article
  6. Review
  7. Review
  8. Review
  9. Article
  10. Review
  11. Review
  12. Article
  13. Article
  14. Article
  15. Review
  16. Point of care testing for infectious diseases.Clinica chimica acta; international journal of clinical chemistry · 2019
    Review
  17. Article
  18. The current landscape of nucleic acid tests for filovirus detection.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2018
    Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Jason W BenzineLucigen Corp., Middleton, Wisconsin.
Kerry M BrownLucigen Corp., Middleton, Wisconsin.
Krystle N AgansDepartment of Microbiology and Immunology Galveston National Laboratory, University of Texas Medical Branch at Galveston.
Ronald GodiskaLucigen Corp., Middleton, Wisconsin.
Chad E MireDepartment of Microbiology and Immunology Galveston National Laboratory, University of Texas Medical Branch at Galveston.
Krishne GowdaLucigen Corp., Middleton, Wisconsin.
Brandon ConverseLucigen Corp., Middleton, Wisconsin.
Thomas W GeisbertDepartment of Microbiology and Immunology Galveston National Laboratory, University of Texas Medical Branch at Galveston.
David A MeadLucigen Corp., Middleton, Wisconsin.
Yogesh ChanderLucigen Corp., Middleton, Wisconsin.
Lucigen Corporation · USThe University of Texas Medical Branch at Galveston · US

Funding

Regulatory Compliance Core - Galveston National Laboratory BSL4 OperationsUC7AI094660 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI Dennis A Bente · 2011 to 2026
$208.8M
Rapid Field Appropriate Diagnostics of Select Emerging and Biodefense PathogensR33AI100182 · NIAID · LUCIGEN CORPORATION · PI MANNA, DIPANKAR · 2014 to 2016
$1.7M
Rapid Field Appropriate Diagnostics for Select Biodefense and Emerging PathogensR21AI100182 · NIAID · LUCIGEN CORPORATION · PI CHANDER, YOGESH · 2012 to 2013
$441k
NIAID NIH HHS R21 AI100182NIAID NIH HHS R33 AI100182NIAID NIH HHS UC7 AI094660
6 · The paper itself

Abstract

A molecular diagnostic method for robust detection of Ebola virus (EBOV) at the point of care (POC) directly from blood samples is described. This assay is based on reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) of the glycoprotein gene of EBOV. Complete reaction formulations were lyophilized in 0.2-mL polymerase chain reaction tubes. RT-LAMP reactions were performed on a battery-operated isothermal instrument. Limit of detection of this RT-LAMP assay was 2.8 × 10

Indexed as

Point-of-Care SystemsEbolavirusHemorrhagic Fever, EbolaHumansMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesRNA, ViralSensitivity and SpecificityRNA, Viraldiagnostic testEbola virusisothermal amplificationpoint of careRT-LAMP

Identifiers

PMID27638947
PMCPMC5050483
OpenAlexW2518710013

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.