ArticleEnvironmental science and pollution research international2016
Occurrence of Giardia intestinalis and Cryptosporidium sp. in wastewater samples from São Paulo State, Brazil, and Lima, Peru.
Article in Environmental science and pollution research international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
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- Wastewater surveillance beyond COVID-19: a ranking system for communicable disease testing in the tri-county Detroit area, Michigan, USA.Frontiers in public health · 2023Article
- Burden and Epidemiology of Human IntestinalTropical medicine and infectious disease · 2022Review
- Detection ofMicroorganisms · 2022Review
- Molecular Detection ofVeterinary sciences · 2022Article
- Wastewater-based epidemiology-surveillance and early detection of waterborne pathogens with a focus on SARS-CoV-2, Cryptosporidium and Giardia.Parasitology research · 2021Review
- Multilocus Genotyping ofPathogens (Basel, Switzerland) · 2021Article
- Epidemiology of Giardia duodenalis assemblages in Brazil: there is still a long way to go.Memorias do Instituto Oswaldo Cruz · 2021Review
- Detection and molecular characterization of Cryptosporidium species and Giardia assemblages in two watersheds in the metropolitan region of São Paulo, Brazil.Environmental science and pollution research international · 2018Article
- Occurrence and molecular characterization of Giardia duodenalis cysts and Cryptosporidium oocysts in raw water samples from the Rímac River, Peru.Environmental science and pollution research international · 2018Article
- Molecular epidemiologic tools for waterborne pathogensFood and waterborne parasitologyReview
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Authors and funding
10 authors.
Funding
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Abstract
The objectives of the study were to detect and genotype Cryptosporidium spp. and Giardia intestinalis in wastewater samples obtained from five cities with high transit of people in the State of São Paulo, Brazil, and at the entrance of a Wastewater Treatment Plant (WWTP) in Lima, Peru. Samples were collected and concentrated by centrifugation. The genomic DNA was extracted for molecular characterization by nested PCR for Cryptosporidium and double nested PCR for Giardia, followed by sequencing and phylogenetic analysis. G. intestinalis was found in 63.6 % of the samples, and the human assemblages A and B were identified. Cryptosporidium sp. was found in 36.4 % of the samples, and the species were corresponding to Cryptosporidium hominis, Cryptosporidium cuniculus, and Cryptosporidium muris. Results revealed the presence of human pathogenic Cryptosporidium species and G. intestinalis human pathogenic assemblages. Molecular tools highlight the importance to map the genetic diversity of these parasites, as well as to detect their epidemiological circulation pathway in the environment.
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