ArticleThe Journal of biological chemistry2016
Helicoidal Organization of Chitin in the Cuticle of the Migratory Locust Requires the Function of the Chitin Deacetylase2 Enzyme (LmCDA2).
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 104 citations in OpenAlex.
- Effects of chitin derived from black soldier fly larvae on growth performance, antioxidant capacity, hepatopancreas morphology, intestine microbiota, immune capacity, and lipid metabolism of Pacific white shrimp (Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Article
- Dynamic eIF3-S6 Phase Separation Switch Instructed by mAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The Tra/Dsx-JHBP axis controls female-specific gene expression and oviposition in locusts.PLoS biology · 2025Article
- Nanoscale cuticle mass density variations influenced by pigmentation in butterfly wing scales.Nature communications · 2025Article
- The dual role of the RR-2 cuticular protein gene in development and acaricide susceptibility of Panonychus citri (Acari: Tetranychidae).Experimental & applied acarology · 2025Article
- Group V Chitin Deacetylases Are Responsible for the Structure and Barrier Function of the Gut Peritrophic Matrix in the Chinese Oak SilkwormInternational journal of molecular sciences · 2024Article
- Article
- Group V Chitin Deacetylases Influence the Structure and Composition of the Midgut of Beet Armyworm,International journal of molecular sciences · 2023Article
- Article
- Proteomic analysis of pharate pupal molting fluid from the tobacco hornworm, Manduca sexta.Insect biochemistry and molecular biology · 2022Article
- The genetic basis of structural colour variation in mimeticPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Chitin deacetylases are necessary for insect femur muscle attachment and mobility.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Improving Polysaccharide-Based Chitin/Chitosan-Aerogel Materials by Learning from Genetics and Molecular Biology.Materials (Basel, Switzerland) · 2022Review
- Identification and RNAi-Based Functional Analysis of Four Chitin Deacetylase Genes in Sogatella furcifera (Hemiptera: Delphacidae).Journal of insect science (Online) · 2021Article
- An overall look at insect chitin deacetylases: Promising molecular targets for developing green pesticides.Journal of pesticide science · 2021Article
- A novel tick protein supports integrity of gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens.Cellular microbiology · 2021Article
- Epidermal Cell Surface Structure and Chitin-Protein Co-assembly Determine Fiber Architecture in the Locust Cuticle.ACS applied materials & interfaces · 2020Article
- Insect Mouthpart Transcriptome Unveils Extension of Cuticular Protein Repertoire and Complex Organization.iScience · 2020Article
- Mechanisms, Applications, and Challenges of Insect RNA Interference.Annual review of entomology · 2020Review
- Insect Cuticular Chitin Contributes to Form and Function.Current pharmaceutical design · 2020Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the three-dimensional extracellular matrix of the insect cuticle, horizontally aligned microfibrils composed of the polysaccharide chitin and associated proteins are stacked either parallel to each other or helicoidally. The underlying molecular mechanisms that implement differential chitin organization are largely unknown. To learn more about cuticle organization, we sought to study the role of chitin deacetylases (CDA) in this process. In the body cuticle of nymphs of the migratory locust Locusta migratoria, helicoidal chitin organization is changed to an organization with unidirectional microfibril orientation when LmCDA2 expression is knocked down by RNA interference. In addition, the LmCDA2-deficient cuticle is less compact suggesting that LmCDA2 is needed for chitin packaging. Animals with reduced LmCDA2 activity die at molting, underlining that correct chitin organization is essential for survival. Interestingly, we find that LmCDA2 localizes only to the initially produced chitin microfibrils that constitute the apical site of the chitin stack. Based on our data, we hypothesize that LmCDA2-mediated chitin deacetylation at the beginning of chitin production is a decisive reaction that triggers helicoidal arrangement of subsequently assembled chitin-protein microfibrils.
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