Evidence map›Paper›PMID 27635362›Full record

ArticlePeerJ2016

Characterization, development and multiplexing of microsatellite markers in three commercially exploited reef fish and their application for stock identification.

Laura Taillebois, Christine Dudgeon, Safia Maher, David A Crook, Thor M Saunders, Diane P Barton, Jonathan A Taylor, David J Welch, Stephen J Newman, Michael J Travers and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Laura TailleboisResearch Institute for the Environment and Livelihoods, Charles Darwin University, Darwin, Northern Territory, Australia; North Australia Marine Research Alliance, Arafura Timor Research Facility, Darwin, Northern Territory, Australia.
Christine DudgeonMolecular Fisheries Laboratory, School of Biomedical Sciences, University of Queensland , Brisbane , Queensland , Australia.
Safia MaherMolecular Fisheries Laboratory, School of Biomedical Sciences, University of Queensland , Brisbane , Queensland , Australia.
David A CrookResearch Institute for the Environment and Livelihoods, Charles Darwin University , Darwin , Northern Territory , Australia.
Thor M SaundersFisheries Research, Northern Territory Department of Primary Industries and Fisheries , Berrimah , Northern Territory , Australia.
Diane P BartonResearch Institute for the Environment and Livelihoods, Charles Darwin University, Darwin, Northern Territory, Australia; Fisheries Research, Northern Territory Department of Primary Industries and Fisheries, Berrimah, Northern Territory, Australia.
Jonathan A TaylorFisheries Research, Northern Territory Department of Primary Industries and Fisheries , Berrimah , Northern Territory , Australia.
David J WelchC2O Fisheries , Cairns , Queensland , Australia.
Stephen J NewmanWestern Australian Fisheries and Marine Research Laboratories, Department of Fisheries, Government of Western Australia , North Beach , Western Australia , Australia.
Michael J TraversWestern Australian Fisheries and Marine Research Laboratories, Department of Fisheries, Government of Western Australia , North Beach , Western Australia , Australia.
Richard J SaundersCentre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Douglas, Queensland, Australia; Animal Science, Queensland Department of Agriculture and Fisheries, Brisbane, Queensland, Australia.
Jennifer OvendenMolecular Fisheries Laboratory, School of Biomedical Sciences, University of Queensland , Brisbane , Queensland , Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Thirty-four microsatellite loci were isolated from three reef fish species; golden snapper Lutjanus johnii, blackspotted croaker Protonibea diacanthus and grass emperor Lethrinus laticaudis using a next generation sequencing approach. Both IonTorrent single reads and Illumina MiSeq paired-end reads were used, with the latter demonstrating a higher quality of reads than the IonTorrent. From the 1-1.5 million raw reads per species, we successfully obtained 10-13 polymorphic loci for each species, which satisfied stringent design criteria. We developed multiplex panels for the amplification of the golden snapper and the blackspotted croaker loci, as well as post-amplification pooling panels for the grass emperor loci. The microsatellites characterized in this work were tested across three locations of northern Australia. The microsatellites we developed can detect population differentiation across northern Australia and may be used for genetic structure studies and stock identification.

Indexed as

Lethrinus laticaudisLutjanus johniiMicrosatellite markersMultiplex panelsNext-generation sequencingProtonibea diacanthus

Identifiers

PMID27635362
PMCPMC5012413

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.