ArticleGenome biology2016
An alternative novel tool for DNA editing without target sequence limitation: the structure-guided nuclease.
Article in Genome biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 38 citations in OpenAlex.
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- Expanding the CRISPR Toolbox in Zebrafish for Studying Development and Disease.Frontiers in cell and developmental biology · 2019Review
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- HPV Oncogene Manipulation Using Nonvirally Delivered CRISPR/Cas9 orAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Genome Editing of Pigs for Agriculture and Biomedicine.Frontiers in genetics · 2018Review
- Zebrafish Znfl1 proteins control the expression ofThe Journal of biological chemistry · 2017Article
- Modern Genome Editing Technologies in Huntington's Disease Research.Journal of Huntington's disease · 2017Review
- Programmable Genome Editing Tools and their Regulation for Efficient Genome Engineering.Computational and structural biotechnology journal · 2017Review
- DNA-guided genome editing using structure-guided endonucleases.Genome biology · 2016Article
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Engineered endonucleases are a powerful tool for editing DNA. However, sequence preferences may limit their application. We engineer a structure-guided endonuclease (SGN) composed of flap endonuclease-1 (FEN-1), which recognizes the 3' flap structure, and the cleavage domain of Fok I (Fn1), which cleaves DNA strands. The SGN recognizes the target DNA on the basis of the 3' flap structure formed between the target and the guide DNA (gDNA) and cut the target through its Fn1 dimerization. Our results show that the SGN, guided by a pair of gDNAs, cleaves transgenic reporter gene and endogenous genes in zebrafish embryonic genome.
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