ArticleScientific reports2016
External cavity-quantum cascade laser infrared spectroscopy for secondary structure analysis of proteins at low concentrations.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 79 citations in OpenAlex.
- Broadband comb spectroscopy through spectral envelope shaping.Nature communications · 2026Article
- Label-free protein-structure-sensitive live-cell microscopy for patient-specific assessment of myeloma therapy.Nature biomedical engineering · 2026Article
- External Cavity Quantum Cascade Laser Vibrational Circular Dichroism Spectroscopy for Fast and Sensitive Analysis of Proteins at Low Concentrations.Analytical chemistry · 2024Article
- Ultrathin Ge-YFNanophotonics (Berlin, Germany) · 2024Article
- Analytical approaches for assessing protein structure in protein-rich food: A comprehensive review.Food chemistry: X · 2024Review
- Laser-Based Mid-Infrared Spectroscopy for Monitoring Temperature-Induced Denaturation of Bovine Serum Albumin and De-/Stabilization Effects of Sugars.Analytical chemistry · 2023Article
- A mid-infrared lab-on-a-chip for dynamic reaction monitoring.Nature communications · 2022Article
- QCL-IR Spectroscopy for In-Line Monitoring of Proteins from Preparative Ion-Exchange Chromatography.Analytical chemistry · 2022Article
- Compensation of Strong Water Absorption in Infrared Spectroscopy Reveals the Secondary Structure of Proteins in Dilute Solutions.Analytical chemistry · 2021Article
- Polarimetric Balanced Detection: Background-Free Mid-IR Evanescent Field Laser Spectroscopy for Low-Noise, Long-term Stable Chemical Sensing.ACS sensors · 2021Article
- The Next Generation of IR Spectroscopy: EC-QCL-Based Mid-IR Transmission Spectroscopy of Proteins with Balanced Detection.Analytical chemistry · 2020Article
- Swept-wavelength lasers based on GaSb gain-chip technology for non-invasive biomedical sensing applications in the 1.7-2.5 μm wavelength range.Biomedical optics express · 2018Article
- Real-Time In Situ Secondary Structure Analysis of Protein Monolayer with Mid-Infrared Plasmonic Nanoantennas.ACS sensors · 2018Article
- Infrared Spectroscopic Imaging Advances as an Analytical Technology for Biomedical Sciences.Analytical chemistry · 2018Review
- Teaching an old pET new tricks: tuning of inclusion body formation and properties by a mixed feed system in E. coli.Applied microbiology and biotechnology · 2018Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fourier transform infrared (FTIR) and circular dichroism (CD) spectroscopy are analytical techniques employed for the analysis of protein secondary structure. The use of CD spectroscopy is limited to low protein concentrations (<2 mg ml(-1)), while FTIR spectroscopy is commonly used in a higher concentration range (>5 mg ml(-1)). Here we introduce a quantum cascade laser (QCL)-based IR transmission setup for analysis of protein and polypeptide secondary structure at concentrations as low as 0.25 mg ml(-1) in deuterated buffer solution. We present dynamic QCL-IR spectra of the temperature-induced α-helix to β-sheet transition of poly-L-lysine. The concentration dependence of the α-β transition temperature between 0.25 and 10 mg ml(-1) was investigated by QCL-IR, FTIR and CD spectroscopy. By using QCL-IR spectroscopy it is possible to perform IR spectroscopic analysis in the same concentration range as CD spectroscopy, thus enabling a combined analysis of biomolecules secondary structure by CD and IR spectroscopy.
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