ArticleScientific reports2016
Autophagy-related proteins are functionally active in human spermatozoa and may be involved in the regulation of cell survival and motility.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 115 citations in OpenAlex.
- Review
- The mitochondrial signature of sperm motility loss: a study of mitophagy and apoptosis in asthenozoospermia.Journal of assisted reproduction and genetics · 2026Article
- Regulation of mitochondrial function during spermatogenesis and sperm maturation.Cellular & molecular biology letters · 2026Review
- Oxidative Stress-Related Programmed Cell Death in Male Infertility: Focussing on Ferroptosis.Journal of cellular and molecular medicine · 2026Review
- Autophagy-Related Proteins Influence Mouse Epididymal Sperm Motility.International journal of molecular sciences · 2025Article
- The role of granulocyte-macrophage colony-stimulating factor in inducing autophagy in the spermatozoa of patients with asthenoteratozoospermia.Clinical and experimental reproductive medicine · 2025Article
- [Molecular Mechanisms of Huangjing Zanyu Capsule in Treating Oligoasthenospermia: A Study Based on AMPK-Mediated Mitophagy].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2025Article
- Genome-Wide microRNA Expression Profiling in Human Spermatozoa and Its Relation to Sperm Quality.Genes · 2025Article
- EnterohemorrhagicAIMS microbiology · 2025Article
- Ultrastructural evidence for the activation of autophagy and analysis of the protective role of autophagy in goat spermatozoa under liquid storage.Frontiers in veterinary science · 2025Article
- Treatment with Pterostilbene Ameliorates the Antioxidant Status of Bovine Spermatozoa and Modulates Cell Death Pathways.Antioxidants (Basel, Switzerland) · 2024Article
- Revisiting cadmium-induced toxicity in the male reproductive system: an update.Archives of toxicology · 2024Review
- c-Jun N-terminal Kinase Supports Autophagy in Testicular Ischemia but Triggers Apoptosis in Ischemia-Reperfusion Injury.International journal of molecular sciences · 2024Article
- Mitophagy and spermatogenesis: Role and mechanisms.Biochemistry and biophysics reports · 2024Review
- Mitophagy in spermatogenesis: Roles and mechanisms.Biochemistry and biophysics reports · 2024Article
- Thymoquinone effects on autophagy, apoptosis, and oxidative stress in cisplatin-induced testicular damage in mice.Journal of assisted reproduction and genetics · 2024Article
- Sperm freezing damage: the role of regulated cell death.Cell death discovery · 2024Review
- The constructive and destructive impact of autophagy on both genders' reproducibility, a comprehensive review.Autophagy · 2023Review
- Autophagy, a critical element in the aging male reproductive disorders and prostate cancer: a therapeutic point of view.Reproductive biology and endocrinology : RB&E · 2023Review
- Male obesity is associated with sperm telomere shortening and aberrant mRNA expression of autophagy-related genes.Basic and clinical andrology · 2023Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Macroautophagy (hereafter autophagy) is an evolutionarily highly conserved cellular process that participates in the maintenance of intracellular homeostasis through the degradation of most long-lived proteins and entire organelles. Autophagy participates in some reproductive events; however, there are not reports regarding the role of autophagy in the regulation of sperm physiology. Hence, the aim of this study was to investigate whether autophagy-related proteins are present and functionally active in human spermatozoa. Proteins related to autophagy/mitophagy process (LC3, Atg5, Atg16, Beclin 1, p62, m-TOR, AMPKα 1/2, and PINK1) were present in human spermatozoa. LC3 colocalized with p62 in the middle piece of the spermatozoa. Autophagy activation induced a significant increase in motility and a decrease in PINK1, TOM20 expression and caspase 3/7 activation. In contrast, autophagy inhibition resulted in decreased motility, viability, ATP and intracellular calcium concentration whereas PINK1, TOM20 expression, AMPK phosphorylation and caspase 3/7 activation were significantly increased. In conclusion our results show that autophagy related proteins and upstream regulators are present and functional in human spermatozoa. Modification of mitochondrial proteins expression after autophagy activation/inhibition may be indicating that a specialized form of autophagy named mitophagy may be regulating sperm function such as motility and viability and may be cooperating with apoptosis.
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