Evidence map›Paper›PMID 27632283›Full record

ArticleVirus genes2017

Iranian johnsongrass mosaic virus: the complete genome sequence, molecular and biological characterization, and comparison of coat protein gene sequences.

Zohreh Moradi, Mohsen Mehrvar, Ehsan Nazifi, Mohammad Zakiaghl

Abstract read
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Article in Virus genes, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.7field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 17 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Zohreh MoradiDepartment of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 1163, 91779-48974, Mashhad, Iran.
Mohsen MehrvarDepartment of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 1163, 91779-48974, Mashhad, Iran. mehrvar@um.ac.ir.
Ehsan NazifiDepartment of Biology, Faculty of Basic Sciences, University of Mazandaran, P.O. Box 47416-95447, 47416-13534, Babolsar, Mazandaran, Iran.
Mohammad ZakiaghlDepartment of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 1163, 91779-48974, Mashhad, Iran.
Ferdowsi University of Mashhad · IRUniversity of Mazandaran · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Iranian johnsongrass mosaic virus (IJMV) is one of the most prevalent viruses causing maize mosaic disease in Iran. An IJMV isolate, Maz-Bah, was obtained from the maize showing mosaic symptoms in Mazandaran, north of Iran. The complete genomic sequence of Maz-Bah is 9544 nucleotides, excluding the poly(A) tail. It contains one single open reading frame of 9165 nucleotides and encodes a large polyprotein of 3054 amino acids, flanked by a 5'-untranslated region (UTR) of 143 nucleotides and a 3'-UTR of 236 nucleotides. The entire genomic sequence of Maz-Bah isolate shares identities of 84.9 and 94.2 % with the IJMV (Shz) isolate, the lone complete genome sequence available in the GenBank at the nucleotide (nt) and deduced amino acid (aa) levels, respectively. The whole genome sequences share identities of 51.5-69.8 and 44.9-74.3 % with those of other Sugarcane mosaic virus (SCMV) subgroup potyviruses at nt and aa levels, respectively. In phylogenetic trees based on the multiple alignments of the entire nt and aa sequences, IJMV isolates formed a separate sublineage of the tree with potyviruses infecting monocotyledons of cereals, indicating that IJMV is a member of SCMV subgroup of potyviruses. IJMV is most closely related to Sorghum mosaic virus and Maize dwarf mosaic virus and less closely related to the Johnsongrass mosaic virus and Cocksfoot streak virus. To further investigate the genetic relationship of IJMV, 9 other isolates from different hosts were cloned and sequenced. The identity of IJMV CP nt and aa sequences of 11 Iranian isolates ranged from 86.4 to 99.8 % and 90.5 to 99.7 %, respectively, indicating a high nt variability in CP gene. Furthermore, in the CP-based phylogenetic tree, IJMV isolates were clustered together with a maize potyvirus described as Zea mosaic virus from Israel (with 86-89 % nt identity), indicating that both isolates probably are the strains of the same virus.

Indexed as

Genome, Viral3' Untranslated Regions5' Untranslated RegionsCapsid ProteinsEvolution, MolecularGenomicsHost SpecificityIranOpen Reading FramesPhylogenyPlant DiseasesPotyvirusSelection, GeneticSequence Analysis, DNAZea mays3' Untranslated Regions5' Untranslated RegionsCapsid ProteinsCoat protein geneComplete genomeIJMVPhylogenetic analysisPotyvirus

Identifiers

PMID27632283
OpenAlexW2515202318

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.