ArticleTransgenic research2016
Comparison of droplet digital PCR with quantitative real-time PCR for determination of zygosity in transgenic maize.
Article in Transgenic research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Epichloë fungal endophyte interactions in perennial ryegrass (Lolium perenne L.) modified to accumulate foliar lipids for increased energy density.BMC plant biology · 2023Article
- Article
- An accurate, reliable, and universal qPCR method to identify homozygous single insert T-DNA with the example of transgenic rice.Frontiers in plant science · 2023Article
- High-Throughput and Accurate Determination of Transgene Copy Number and Zygosity in Transgenic Maize: From DNA Extraction to Data Analysis.International journal of molecular sciences · 2021Article
- Resistance to Aflatoxin Accumulation in Maize Mediated by Host-Induced Silencing of theJournal of fungi (Basel, Switzerland) · 2021Article
- A Strategy for the Production and Molecular Validation of Agrobacterium-Mediated Intragenic Octoploid Strawberry.Plants (Basel, Switzerland) · 2021Article
- Development and assessment of a duplex droplet digital PCR method for quantification of GM rice Kemingdao.Analytical and bioanalytical chemistry · 2021Article
- Digital PCR (dPCR) and qPCR mediated determination of transgene copy number in the forage legume white clover (Trifolium repens).Molecular biology reports · 2021Article
- Evolution of the Brassicaceae-specific MS5-Like family and neofunctionalization of the novel MALE STERILITY 5 gene essential for male fertility in Brassica napus.The New phytologist · 2021Article
- Identification of Loci Enabling Stable and High-Level Heterologous Gene Expression.Frontiers in bioengineering and biotechnology · 2021Article
- Digital PCR: What Relevance to Plant Studies?Biology · 2020Review
- Host Induced Gene Silencing TargetingFrontiers in microbiology · 2020Article
- Structural and Functional Analysis of a Bidirectional Promoter fromInternational journal of molecular sciences · 2018Article
- Comparative analysis of miRNA expression profiles in transgenic and non-transgenic rice using miRNA-Seq.Scientific reports · 2018Article
- Development and Application of Droplet Digital PCR Tools for the Detection of Transgenes in Pastures and Pasture-Based Products.Frontiers in plant science · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study evaluated the applicability of droplet digital PCR (ddPCR) as a tool for maize zygosity determination using quantitative real-time PCR (qPCR) as a reference technology. Quantitative real-time PCR is commonly used to determine transgene copy number or GMO zygosity characterization. However, its effectiveness is based on identical reaction efficiencies for the transgene and the endogenous reference gene. Additionally, a calibrator sample should be utilized for accuracy. Droplet digital PCR is a DNA molecule counting technique that directly counts the absolute number of target and reference DNA molecules in a sample, independent of assay efficiency or external calibrators. The zygosity of the transgene can be easily determined using the ratio of the quantity of the target gene to the reference single copy endogenous gene. In this study, both the qPCR and ddPCR methods were used to determine insect-resistant transgenic maize IE034 zygosity. Both methods performed well, but the ddPCR method was more convenient because of its absolute quantification property.
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