ArticleFrontiers in molecular biosciences2016
The Influence of Copy-Number of Targeted Extrachromosomal Genetic Elements on the Outcome of CRISPR-Cas Defense.
Article in Frontiers in molecular biosciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Comprehensive analysis of the CRISPR-Cas systems in Streptococcus thermophilus strains isolated from traditional yogurts.Antonie van Leeuwenhoek · 2024Article
- Persistence of plasmids targeted by CRISPR interference in bacterial populations.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Single cell variability of CRISPR-Cas interference and adaptation.Molecular systems biology · 2022Article
- Primed adaptation tolerates extensive structural and size variations of the CRISPR RNA guide in Haloarcula hispanica.Nucleic acids research · 2019Article
- Systematic analysis of Type I-E Escherichia coli CRISPR-Cas PAM sequences ability to promote interference and primed adaptation.Molecular microbiology · 2019Article
- Molecular mechanisms of CRISPR-Cas spacer acquisition.Nature reviews. Microbiology · 2019Review
- Role of nucleotide identity in effective CRISPR target escape mutations.Nucleic acids research · 2018Article
- Article
- Primed CRISPR adaptation in Escherichia coli cells does not depend on conformational changes in the Cascade effector complex detected in Vitro.Nucleic acids research · 2018Article
- Article
- Optimal number of spacers in CRISPR arrays.PLoS computational biology · 2017Article
- Priming in a permissive type I-C CRISPR-Cas system reveals distinct dynamics of spacer acquisition and loss.RNA (New York, N.Y.) · 2017Article
- Features of CRISPR-Cas Regulation Key to Highly Efficient and Temporally-Specific crRNA Production.Frontiers in microbiology · 2017Article
- Altered stoichiometry Escherichia coli Cascade complexes with shortened CRISPR RNA spacers are capable of interference and primed adaptation.Nucleic acids research · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 3 countries.
Funding
Abstract
Prokaryotic type I CRISPR-Cas systems respond to the presence of mobile genetic elements such as plasmids and phages in two different ways. CRISPR interference efficiently destroys foreign DNA harboring protospacers fully matching CRISPR RNA spacers. In contrast, even a single mismatch between a spacer and a protospacer can render CRISPR interference ineffective but causes primed adaptation-efficient and specific acquisition of additional spacers from foreign DNA into the CRISPR array of the host. It has been proposed that the interference and primed adaptation pathways are mediated by structurally different complexes formed by the effector Cascade complex on matching and mismatched protospacers. Here, we present experimental evidence and present a simple mathematical model that shows that when plasmid copy number maintenance/phage genome replication is taken into account, the two apparently different outcomes of the CRISPR-Cas response can be accounted for by just one kind of effector complex on both targets. The results underscore the importance of consideration of targeted genome biology when considering consequences of CRISPR-Cas systems action.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.