ArticleFrontiers in plant science2016
Differential Contribution of the First Two Enzymes of the MEP Pathway to the Supply of Metabolic Precursors for Carotenoid and Chlorophyll Biosynthesis in Carrot (Daucus carota).
Article in Frontiers in plant science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 76 citations in OpenAlex.
- Beyond Tanacetum cinerariifolium: Advances in Pyrethrins Biosynthesis and Prospects for Engineered Cell Factories.Molecular biotechnology · 2026Review
- The Association Between the Flesh Colour and Carotenoid Profile of 25 Cultivars of Mangoes.Molecules (Basel, Switzerland) · 2025Article
- Exploring the Potential of Carotenoids in Obesity Management: A Comprehensive Overview.Recent advances in food, nutrition & agriculture · 2025Review
- The best of both worlds: photosynthesis and Solanaceae biodiversity seeking a sustainable food and cosmetic industry.Frontiers in plant science · 2024Article
- 3-Hydroxy-3-methylglutaryl coenzyme A reductase genes from Glycine max regulate plant growth and isoprenoid biosynthesis.Scientific reports · 2023Article
- High-quality genome assembly and genetic mapping reveal a gene regulating flesh color in watermelon (Frontiers in plant science · 2023Article
- Overexpression ofForestry research · 2023Article
- Differential roles ofFrontiers in plant science · 2023Article
- Comparative transcriptomic and plastid development analysis sheds light on the differential carotenoid accumulation in kiwifruit flesh.Frontiers in plant science · 2023Article
- Identification of SNP loci and candidate genes genetically controlling norisoprenoids in grape berry based on genome-wide association study.Frontiers in plant science · 2023Article
- Carrot DcALFIN4 and DcALFIN7 Transcription Factors Boost Carotenoid Levels and Participate Differentially in Salt Stress Tolerance When Expressed inInternational journal of molecular sciences · 2022Article
- Carotenoids in Drug Discovery and Medicine: Pathways and Molecular Targets Implicated in Human Diseases.Molecules (Basel, Switzerland) · 2022Review
- Development and carotenoid synthesis in dark-grown carrot taproots require PHYTOCHROME RAPIDLY REGULATED1.Plant physiology · 2022Article
- Article
- Riboswitch-mediated inducible expression of an astaxanthin biosynthetic operon in plastids.Plant physiology · 2022Article
- Characterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation.PloS one · 2022Article
- Inhibition of Carotenoid Biosynthesis by CRISPR/Cas9 Triggers Cell Wall Remodelling in Carrot.International journal of molecular sciences · 2021Article
- The Genetic Basis of Tomato Aroma.Genes · 2021Review
- A de novo transcriptome analysis revealed that photomorphogenic genes are required for carotenoid synthesis in the dark-grown carrot taproot.Molecular genetics and genomics : MGG · 2020Article
- The organ-specific differential roles of rice DXS and DXR, the first two enzymes of the MEP pathway, in carotenoid metabolism in Oryza sativa leaves and seeds.BMC plant biology · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carotenoids and chlorophylls are photosynthetic pigments synthesized in plastids from metabolic precursors provided by the methylerythritol 4-phosphate (MEP) pathway. The first two steps in the MEP pathway are catalyzed by the deoxyxylulose 5-phosphate synthase (DXS) and reductoisomerase (DXR) enzymes. While DXS has been recently shown to be the main flux-controlling step of the MEP pathway, both DXS and DXR enzymes have been proven to be able to promote an increase in MEP-derived products when overproduced in diverse plant systems. Carrot (Daucus carota) produces photosynthetic pigments (carotenoids and chlorophylls) in leaves and in light-exposed roots, whereas only carotenoids (mainly α- and β-carotene) accumulate in the storage root in darkness. To evaluate whether DXS and DXR activities influence the production of carotenoids and chlorophylls in carrot leaves and roots, the corresponding Arabidopsis thaliana genes were constitutively expressed in transgenic carrot plants. Our results suggest that DXS is limiting for the production of both carotenoids and chlorophylls in roots and leaves, whereas the regulatory role of DXR appeared to be minor. Interestingly, increased levels of DXS (but not of DXR) resulted in higher transcript abundance of endogenous carrot genes encoding phytoene synthase, the main rate-determining enzyme of the carotenoid pathway. These results support a central role for DXS on modulating the production of MEP-derived precursors to synthesize carotenoids and chlorophylls in carrot, confirming the pivotal relevance of this enzyme to engineer healthier, carotenoid-enriched products.
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