ArticleFrontiers in microbiology2016
Simple and Versatile Turbidimetric Monitoring of Bacterial Growth in Liquid Cultures Using a Customized 3D Printed Culture Tube Holder and a Miniaturized Spectrophotometer: Application to Facultative and Strictly Anaerobic Bacteria.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
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- Two marine sulfur-reducing bacteria co-culture is essential for productive infection by a T4-likeHeliyon · 2024Article
- Toward the Real-Time and Rapid Quantification of Bacterial Cells Utilizing a Quartz Tuning Fork Sensor.Micromachines · 2023Article
- Pilot Testing of the "Turbidimeter", a Simple, Universal Reader Intended to Complement and Enhance Bacterial Growth Detection in Manual Blood Culture Systems in Low-Resource Settings.Diagnostics (Basel, Switzerland) · 2022Article
- Low-Cost Clamp-On Photometers (ClampOD) and Tube Photometers (TubeOD) for Online Cell Density Determination.Frontiers in microbiology · 2021Article
- High resolution microscopy to evaluate the efficiency of surface sterilization of Zea Mays seeds.PloS one · 2020Article
- Performance of a Newly Isolated Salt-Tolerant Yeast StrainFrontiers in microbiology · 2020Article
- 3D-printed Bioreactors forInternational journal of bioprinting · 2020Review
- A Real-Time Multiplexed Microbial Growth Intervalometer for Capturing High-Resolution Growth Curves.Frontiers in microbiology · 2019Article
- Impact of bacterial streamers on biofouling of microfluidic filtration systems.Biomicrofluidics · 2018Article
- Integrated Optical Mach-Zehnder Interferometer Based on Organic-Inorganic Hybrids for Photonics-on-a-Chip Biosensing Applications.Sensors (Basel, Switzerland) · 2018Article
- A rapid and low-cost estimation of bacteria counts in solution using fluorescence spectroscopy.Analytical and bioanalytical chemistry · 2017Article
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Authors and funding
7 authors.
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Abstract
Here we introduce a novel strategy for turbidimetric monitoring of bacterial growth in liquid culture. The instrumentation comprises a light source, a customized 3D printed culture tube holder and a miniaturized spectrophotometer, connected through optical cables. Due to its small footprint and the possibility to operate with external light, bacterial growth was directly monitored from culture tubes in a simple and versatile fashion. This new portable measurement technique was used to monitor the growth of facultative (Escherichia coli ATCC/25922, and Staphylococcus aureus ATCC/29213) and strictly (Butyrivibrio fibrisolvens JW11, Butyrivibrio proteoclasticus P18, and Propionibacterium acnes DSMZ 1897) anaerobic bacteria. For E. coli and S. aureus, the growth rates calculated from normalized optical density values were compared with those ones obtained using a benchtop spectrophotometer without significant differences (P = 0.256). For the strictly anaerobic species, a high precision (relative standard deviation < 3.5%) was observed between replicates up to 48 h. Regarding its potential for customization, this manifold could accommodate further developments for customized turbidimetric monitoring, such as the use of light-emitting diodes as a light source or flow cells.
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