ArticleFrontiers in microbiology2016
DNA Phosphorothioate Modification Plays a Role in Peroxides Resistance in Streptomyces lividans.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Pooled it
- Phosphorothioate DNA modification by BREX type 4 systems in the human gut microbiome.Nature communications · 2026Article
- Temporal dynamics and metagenomics of phosphorothioate epigenomes in the human gut microbiome.Microbiome · 2025Article
- PT-seq: A method for metagenomic analysis of phosphorothioate epigenetics in complex microbial communities.bioRxiv : the preprint server for biology · 2024Article
- Temporal dynamics and metagenomics of phosphorothioate epigenomes in the human gut microbiome.bioRxiv : the preprint server for biology · 2024Article
- Efficient production of α-ketoglutaric acid using an economical double-strain cultivation and catalysis system.Applied microbiology and biotechnology · 2023Article
- Phosphorothioate-DNA bacterial diet reduces the ROS levels in C. elegans while improving locomotion and longevity.Communications biology · 2021Article
- The origin and impeded dissemination of the DNA phosphorothioation system in prokaryotes.Nature communications · 2021Article
- The Multifaceted Bacterial Cysteine Desulfurases: From Metabolism to Pathogenesis.Antioxidants (Basel, Switzerland) · 2021Review
- Phosphorothioated DNA Is Shielded from Oxidative Damage.Applied and environmental microbiology · 2019Article
- DNA phosphorothioate modification-a new multi-functional epigenetic system in bacteria.FEMS microbiology reviews · 2019Review
- Natural Products Containing 'Rare' Organophosphorus Functional Groups.Molecules (Basel, Switzerland) · 2019Review
- Structural basis for the recognition of sulfur in phosphorothioated DNA.Nature communications · 2018Article
- Comparative genomics analysis of Clostridium difficile epidemic strain DH/NAP11/106.Microbes and infection · 2018Article
- Occurrence, evolution, and functions of DNA phosphorothioate epigenetics in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Regulation ofFrontiers in microbiology · 2018Article
- Characterization of Sigma Factor Genes inFrontiers in microbiology · 2018Article
- Mechanistic Investigation on ROS Resistance of Phosphorothioated DNA.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA phosphorothioation, conferred by dnd genes, was originally discovered in the soil-dwelling bacterium Streptomyces lividans, and thereafter found to exist in various bacterial genera. However, the physiological significance of this sulfur modification of the DNA backbone remains unknown in S. lividans. Our studies indicate that DNA phosphorothioation has a major role in resistance to oxidative stress in the strain. Although Streptomyces species express multiple catalase/peroxidase and organic hydroperoxide resistance genes to protect them against peroxide damage, a wild type strain of S. lividans exhibited two-fold to 10-fold higher survival, compared to a dnd (-) mutant, following treatment with peroxides. RNA-seq experiments revealed that, catalase and organic hydroperoxide resistance gene expression were not up-regulated in the wild type strain, suggesting that the resistance to oxidative stress was not due to the up-regulation of these genes by DNA phosphorothioation. Quantitative RT-PCR analysis was conducted to trace the expression of the catalase and the organic hydroperoxide resistance genes after peroxides treatments. A bunch of these genes were activated in the dnd (-) mutant rather than the wild type strain in response to peroxides. Moreover, the organic hydroperoxide peracetic acid was scavenged more rapidly in the presence than in the absence of phosphorothioate modification, both in vivo and in vitro. The dnd gene cluster can be up-regulated by the disulfide stressor diamide. Overall, our observations suggest that DNA phosphorothioate modification functions as a peroxide resistance system in S. lividans.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.