ArticleScientific reports2016
Fine-tuning of amino sugar homeostasis by EIIA(Ntr) in Salmonella Typhimurium.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Bacterial nitrogen-related phosphotransferase systems: new insights into diverse roles in virulence, physiology, and central metabolism.Journal of bacteriology · 2026Review
- Bacterial metabolism and susceptibility to cell wall-active antibiotics.Advances in microbial physiology · 2023Review
- The role of nitrogen-responsive regulators in controlling inorganic polyphosphate synthesis inMicrobiology (Reading, England) · 2022Article
- Phyllosphere microbial community of cigar tobacco and its corresponding metabolites.Frontiers in microbiology · 2022Article
- A Nitrogen Metabolic Enzyme ProvidesACS infectious diseases · 2021Article
- Resource sharing between central metabolism and cell envelope synthesis.Current opinion in microbiology · 2021Review
- Atomic-Level Investigation of Reactant Recognition Mechanism and Thermodynamic Property in Glucosamine 6-Phosphate Deaminase Catalysis.Frontiers in chemistry · 2021Article
- Global control of bacterial nitrogen and carbon metabolism by a PTSProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Small RNA-binding protein RapZ mediates cell envelope precursor sensing and signaling in Escherichia coli.The EMBO journal · 2020Article
- Regulation of Iron Uptake by Fine-Tuning the Iron Responsiveness of the Iron Sensor Fur.Applied and environmental microbiology · 2019Article
- Article
- Determination of protein phosphorylation by polyacrylamide gel electrophoresis.Journal of microbiology (Seoul, Korea) · 2019Article
- Retrospective application of transposon-directed insertion-site sequencing to investigate niche-specific virulence of Salmonella Typhimurium in cattle.BMC genomics · 2019Article
- Core non-coding RNAs of Piscirickettsia salmonis.PloS one · 2018Article
- Enzyme IIAScientific reports · 2017Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nitrogen-metabolic phosphotransferase system, PTS(Ntr), consists of the enzymes I(Ntr), NPr and IIA(Ntr) that are encoded by ptsP, ptsO, and ptsN, respectively. Due to the proximity of ptsO and ptsN to rpoN, the PTS(Ntr) system has been postulated to be closely related with nitrogen metabolism. To define the correlation between PTS(Ntr) and nitrogen metabolism, we performed ligand fishing with EIIA(Ntr) as a bait and revealed that D-glucosamine-6-phosphate synthase (GlmS) directly interacted with EIIA(Ntr). GlmS, which converts D-fructose-6-phosphate (Fru6P) into D-glucosamine-6-phosphate (GlcN6P), is a key enzyme producing amino sugars through glutamine hydrolysis. Amino sugar is an essential structural building block for bacterial peptidoglycan and LPS. We further verified that EIIA(Ntr) inhibited GlmS activity by direct interaction in a phosphorylation-state-dependent manner. EIIA(Ntr) was dephosphorylated in response to excessive nitrogen sources and was rapidly degraded by Lon protease upon amino sugar depletion. The regulation of GlmS activity by EIIA(Ntr) and the modulation of glmS translation by RapZ suggest that the genes comprising the rpoN operon play a key role in maintaining amino sugar homeostasis in response to nitrogen availability and the amino sugar concentration in the bacterial cytoplasm.
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