ArticleScientific reports2016
Identification of a novel sesquiterpene biosynthetic machinery involved in astellolide biosynthesis.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 50 citations in OpenAlex.
- Cytochrome P450- and Dehydrogenase-Mediated Regiospecific and Stereoselective Formation of β- and γ-Lactones in Drimane-Type Sesquiterpenoid Biosynthesis.Journal of the American Chemical Society · 2026Article
- Towards a reduced-background cell factory: disruption of a PKS-NRPS hybrid gene (AlepA) abolishing the production of 2-pyridones in Aspergillus oryzae.World journal of microbiology & biotechnology · 2026Article
- Distinct Phenotype and Secondary Metabolite Profile Mark a DominantJournal of fungi (Basel, Switzerland) · 2026Article
- Dimerization-Dependent Trans-Domain Coupling Enables Intermediate Transfer in Fungal Haloacid Dehalogenase-Like Terpene Cyclases.Journal of the American Chemical Society · 2026Article
- Discovery and Biosynthesis of Sesquiterpene Polyketide Ester from an Atypical Terpene and Highly Reducing Polyketide Pathway.JACS Au · 2026Article
- Nitrobenzoyl-insulicolide A: a novel dinitrobenzoyl sesquiterpenoid, induces autophagic cell death in small cell lung cancer cells.Marine life science & technology · 2025Article
- Structural insights into a bacterial terpene cyclase fused with haloacid Dehalogenase-like phosphatase.Chemical science · 2025Article
- Unusual Sesquiterpenes fromJournal of natural products · 2024Article
- Class II terpene cyclases: structures, mechanisms, and engineering.Natural product reports · 2024Review
- Discovery and biosynthesis of bacterial drimane-type sesquiterpenoids fromBeilstein journal of organic chemistry · 2024Article
- Isolation, (bio)synthetic studies and evaluation of antimicrobial properties of drimenol-type sesquiterpenes of Termitomyces fungi.Communications chemistry · 2023Article
- Discovery, Structure, and Mechanism of a Class II Sesquiterpene Cyclase.Journal of the American Chemical Society · 2022Article
- Chemical Diversity and Biosynthesis of Drimane-Type Sesquiterpenes in the Fungal Kingdom.Chembiochem : a European journal of chemical biology · 2022Review
- Identification and Characterization of Bifunctional Drimenol Synthases of Marine Bacterial Origin.ACS chemical biology · 2022Article
- Biosynthesis of Fungal Drimane-Type Sesquiterpene Esters.Angewandte Chemie (International ed. in English) · 2021Article
- Chromolaena laevigata (Asteraceae) as a source of endophytic non-aflatoxigenic Aspergillus flavus: chemical profile in different culture conditions and biological applications.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- cblaster: a remote search tool for rapid identification and visualization of homologous gene clusters.Bioinformatics advances · 2021Article
- Combined Comparative Genomics and Gene Expression Analyses Provide Insights into the Terpene Synthases Inventory inMicroorganisms · 2020Article
- Complete Stereoinversion of l-Tryptophan by a Fungal Single-Module Nonribosomal Peptide Synthetase.Journal of the American Chemical Society · 2019Article
- Taxonomy ofStudies in mycology · 2019Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Esterified drimane-type sesquiterpene lactones such as astellolides display various biological activities and are widely produced by plants and fungi. Given their low homology to known sesquiterpene cyclases, the genes responsible for their biosynthesis have not been uncovered yet. Here, we identified the astellolide gene cluster from Aspergillus oryzae and discovered a novel sesquiterpene biosynthetic machinery consisting of AstC, AstI, and AstK. All these enzymes are annotated as haloacid dehalogenase-like hydrolases, whereas AstC also contains a DxDTT motif conserved in class II diterpene cyclases. Based on enzyme reaction analyses, we found that AstC catalysed the protonation-initiated cyclisation of farnesyl pyrophosphate into drimanyl pyrophosphate. This was successively dephosphorylated by AstI and AstK to produce drim-8-ene-11-ol. Moreover, we also identified and characterised a unique non-ribosomal peptide synthetase, AstA, responsible for esterifying aryl acids to drimane-type sesquiterpene lactones. In this study, we highlight a new biosynthetic route for producing sesquiterpene and its esterified derivative. Our findings shed light on the identification of novel sesquiterpenes via genome mining.
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