ArticleThe Journal of veterinary medical science2017
Development and application of multiplex PCR assays for detection of virus-induced respiratory disease complex in dogs.
Article in The Journal of veterinary medical science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 53 citations in OpenAlex.
- Evaluation of a Point-of-Care Multiplex PCR Analyser for Detection ofVeterinary sciences · 2026Article
- Evidence of feline chaphamaparvovirus in dogs: molecular detection, genetic recombination, and tissue localization.BMC veterinary research · 2025Article
- Development and validation of nanoplate-based RT-dPCR assay for canine respiratory coronavirus detection in various clinical samples.BMC veterinary research · 2025Article
- Automated highly multiplex detection system for respiratory pathogens in canines.Frontiers in veterinary science · 2025Article
- Development of Multiplex PCR assay for detection of Canine Infectious Respiratory Disease Complex (CIRDC) pathogens in dogs.Frontiers in cellular and infection microbiology · 2025Article
- Development of one-step multiplex real-time PCR for the detection of CHV-1, CAdV-2, and CDV.Frontiers in veterinary science · 2025Article
- Evolutionary dynamics of canine kobuvirus in Vietnam and Thailand reveal the evidence of viral ability to evade host immunity.Scientific reports · 2024Article
- Development of a Large-Scale Pathogen Screening Test for the Biosafety Evaluation of Canine Mesenchymal Stem Cells.Biological procedures online · 2023Article
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- Predominance of Canine Parainfluenza Virus andPathogens (Basel, Switzerland) · 2023Article
- Prevalence of canine herpesvirus 1 and associated risk factors among bitches in Iranian breeding kennels and farms.Veterinary medicine and science · 2023Article
- Canine respiratory coronavirus in Thailand undergoes mutation and evidences a potential putative parent for genetic recombination.Microbiology spectrum · 2023Article
- An Improved Duplex Real-Time Quantitative RT-PCR Assay with a Canine Endogenous Internal Positive Control for More Sensitive and Reliable Detection of Canine Parainfluenza Virus 5.Veterinary sciences · 2023Article
- Genetic characterization of canine astrovirus in non-diarrhea dogs and diarrhea dogs in Vietnam and Thailand reveals the presence of a unique lineage.Frontiers in veterinary science · 2023Article
- Epidemiology, genetic diversity, and association of canine circovirus infection in dogs with respiratory disease.Scientific reports · 2022Article
- Detection and molecular characterization of two canine circovirus genotypes co-circulating in Vietnam.The veterinary quarterly · 2021Article
- Review a brief history of coronaviruses in Thailand.Journal of virological methods · 2021Review
- Article
- Molecular and Pathological Investigations of Selected Viral Neuropathogens in Rabies-Negative Brains of Cats and Dogs Revealed Neurotropism of Carnivore Protoparvovirus-1.Frontiers in veterinary science · 2021Article
- Natural infection of parvovirus in wild fishing cats (Prionailurus viverrinus) reveals extant viral localization in kidneys.PloS one · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Canine infectious respiratory disease complex (CIRDC) viruses have been detected in dogs with respiratory illness. Canine influenza virus (CIV), canine parainfluenza virus (CPIV), canine distemper virus (CDV), canine respiratory coronavirus (CRCoV), canine adenovirus type 2 (CAdV-2) and canine herpesvirus 1 (CaHV-1), are all associated with the CIRDC. To allow diagnosis, two conventional multiplex polymerase chain reactions (PCR) were developed to simultaneously identify four RNA and two DNA viruses associated with CIRDC. The two multiplex PCR assays were then validated on 102 respiratory samples collected from 51 dogs with respiratory illness by sensitivity and specificity determination in comparison to conventional simplex PCR and a rapid three-antigen test kit. All six viruses were detected in either individual or multiple infections. The developed multiplex PCR assays had a >87% sensitivity and 100% specificity compared to their simplex counterpart. Compared to the three-antigen test kit, the multiplex PCR assays yielded 100% sensitivity and more than 83% specificity for detection of CAdV-2 and CDV, but not for CIV. Therefore, the developed multiplex PCR modalities were able to simultaneously diagnose a panel of CIRDC viruses and facilitated specimen collection through being suitable for use of nasal or oral samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.