ArticleScientific reports2016
Global analysis of differential gene expression related to long-term sperm storage in oviduct of Chinese Soft-Shelled Turtle Pelodiscus sinensis.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 28 citations in OpenAlex.
- The impact of aquaculture system on the microbiome and gut metabolome of juvenile Chinese softshell turtle (iMeta · 2022Article
- Sperm competition in squamate reptiles.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Article
- Advances in understanding mechanisms of long-term sperm storage-the soft-shelled turtle model.Histology and histopathology · 2020Article
- Role of genome-wide mRNA-seq profiling in understanding the long-term sperm maintenance in the storage tubules of laying hens.Tropical animal health and production · 2019Article
- Genomewide identification and analysis of heat-shock proteins 70/110 to reveal their potential functions in Chinese soft-shelled turtleEcology and evolution · 2019Article
- Autophagy and its link to type II diabetes mellitus.BioMedicine · 2017Article
- Molecular and Cellular Mechanisms of Apoptosis during Dissociated Spermatogenesis.Frontiers in physiology · 2017Article
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9 authors at 1 institution in 1 country.
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Abstract
Important evolutionary and ecological consequences arise from the ability of female turtles to store viable spermatozoa for an extended period. Although previous morphological studies have observed the localization of spermatozoa in Pelodiscus sinensis oviduct, no systematic study on the identification of genes that are involved in long-term sperm storage has been performed. In this study, the oviduct of P. sinensis at different phases (reproductive and hibernation seasons) was prepared for RNA-Seq and gene expression profiling. In total, 2,662 differentially expressed genes (DEGs) including 1,224 up- and 1,438 down-regulated genes were identified from two cDNA libraries. Functional enrichment analysis indicated that many genes were predominantly involved in the immune response, apoptosis pathway and regulation of autophagy. RT-qPCR, ELISA, western blot and IHC analyses showed that the expression profiles of mRNA and protein in selected DEGs were in consistent with results from RNA-Seq analysis. Remarkably, TUNEL analysis revealed the reduced number of apoptotic cells during sperm storage. IHC and TEM analyses found that autophagy occurred in the oviduct epithelial cells, where the spermatozoa were closely attached. The outcomes of this study provide fundamental insights into the complex sperm storage regulatory process and facilitate elucidating the mechanism of sperm storage in P. sinensis.
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