ArticleScientific reports2016
Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 48 citations in OpenAlex.
- The Sperm Acrosomal Reaction Depends on the Function of Both Na,K-ATPase α1 and α4 Isoforms.International journal of molecular sciences · 2026Article
- Glycopolymers Prepared by Alternating Ring-Opening Metathesis Polymerization Provide Access to Distinct, Multivalent Structures for the Probing of Biological Activity.ACS bio & med chem Au · 2024Article
- CaAnimal cells and systems · 2024Review
- Advances in the study of genetic factors and clinical interventions for fertilization failure.Journal of assisted reproduction and genetics · 2023Review
- Hamster Sperm Possess Functional NaInternational journal of molecular sciences · 2023Article
- CatSper Calcium Channels: 20 Years On.Physiology (Bethesda, Md.) · 2023Review
- Mouse sperm energy restriction and recovery (SER) revealed novel metabolic pathways.Frontiers in cell and developmental biology · 2023Article
- Sperm Energy Restriction and Recovery (SER) Alters Epigenetic Marks during the First Cell Cycle of Development in Mice.International journal of molecular sciences · 2022Article
- CatSper and its CaM-like CaFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- C2CD6 regulates targeting and organization of the CatSper calcium channel complex in sperm flagella.Development (Cambridge, England) · 2022Article
- Evolutionary consequences of environmental effects on gamete performance.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- Arrdc4-dependent extracellular vesicle biogenesis is required for sperm maturation.Journal of extracellular vesicles · 2021Article
- Starvation induces an increase in intracellular calcium and potentiates the progesterone-induced mouse sperm acrosome reaction.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Sperm ion channels and transporters in male fertility and infertility.Nature reviews. Urology · 2021Review
- Capacitation increases glucose consumption in murine sperm.Molecular reproduction and development · 2020Article
- A Nonsurgical Embryo Transfer Technique for Fresh and Cultured Blastocysts in Rats.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- Differences and Similarities: The Richness of Comparative Sperm Physiology.Physiology (Bethesda, Md.) · 2020Review
- Zinc exocytosis is sensitive to myosin light chain kinase inhibition in mouse and human eggs.Molecular human reproduction · 2020Article
- Transient Sperm Starvation Improves the Outcome of Assisted Reproductive Technologies.Frontiers in cell and developmental biology · 2019Article
- Only a subpopulation of mouse sperm displays a rapid increase in intracellular calcium during capacitation.Journal of cellular physiology · 2018Article
Corrections and comments
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Authors and funding
13 authors at 5 institutions in 2 countries.
Funding
Abstract
Mammalian sperm acquire fertilizing capacity in the female tract in a process called capacitation. At the molecular level, capacitation requires protein kinase A activation, changes in membrane potential and an increase in intracellular calcium. Inhibition of these pathways results in loss of fertilizing ability in vivo and in vitro. We demonstrated that transient incubation of mouse sperm with Ca(2+) ionophore accelerated capacitation and rescued fertilizing capacity in sperm with inactivated PKA function. We now show that a pulse of Ca(2+) ionophore induces fertilizing capacity in sperm from infertile CatSper1 (Ca(2+) channel), Adcy10 (soluble adenylyl cyclase) and Slo3 (K(+) channel) KO mice. In contrast, sperm from infertile mice lacking the Ca(2+) efflux pump PMACA4 were not rescued. These results indicate that a transient increase in intracellular Ca(2+) can overcome genetic infertility in mice and suggest this approach may prove adaptable to rescue sperm function in certain cases of human male infertility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.