Evidence map›Paper›PMID 27627024›Full record

ArticleJournal of neurogenetics

Acute and chronic effects of clozapine on cholinergic transmission in cultured mouse superior cervical ganglion neurons.

Taixiang Saur, Bruce M Cohen, Qi Ma, Suzann M Babb, Edgar A Buttner, Wei-Dong Yao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neurogenetics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Taixiang Saura McLean Hospital/Harvard Medical School , Belmont , MA , USA.
Bruce M Cohena McLean Hospital/Harvard Medical School , Belmont , MA , USA.
Qi Mab New England Primate Research Center, Harvard Medical School , Southborough , MA , USA.
Suzann M Babba McLean Hospital/Harvard Medical School , Belmont , MA , USA.
Edgar A Buttnera McLean Hospital/Harvard Medical School , Belmont , MA , USA.
Wei-Dong Yaob New England Primate Research Center, Harvard Medical School , Southborough , MA , USA.
Harvard University · USMcLean Hospital · USNew England Biolabs (United States) · US

Funding

New England Primate Research Center Base GrantP51OD011103 · OD · HARVARD MEDICAL SCHOOL · PI FLIER, JEFFREY S · 2012 to 2013
$24.7M
Dopaminergic Enabling of Synaptic Plasticity in Prefrontal CircuitsR01DA032283 · NIDA · UPSTATE MEDICAL UNIVERSITY · PI YAO, WEI-DONG · 2011 to 2015
$1.7M
NIDA NIH HHS R01 DA032283NIH HHS P51 OD011103
6 · The paper itself

Abstract

Cholinergic dysfunction contributes to cognitive deficits in schizophrenia. The atypical antipsychotic clozapine improves cognition in patients with schizophrenia, possibly through modulation of the cholinergic system. However, little is known about specific underlying mechanisms. We investigated the acute and chronic effects of clozapine on cholinergic synaptic transmission in cultured superior cervical ganglion (SCG) neurons. Spontaneous excitatory postsynaptic currents (sEPSCs) were detected and were reversibly inhibited by the nicotinic receptor antagonist d-tubocurarine, confirming that the synaptic responses were primarily mediated by nicotinic receptors. Bath application of clozapine at therapeutic concentrations rapidly and reversely inhibited both the amplitude and frequency of sEPSCs in a concentration-dependent manner, without changing either rise or decay time, suggesting that clozapine effects have both presynaptic and postsynaptic origins. The acute effects of clozapine on sEPSCs were recapitulated by chronic treatment of SCG cultures with similar concentrations of clozapine, as clozapine treatment for 4 d reduced the frequency and amplitude of sEPSCs without affecting their kinetics. Cell survival analysis indicated that SCG neuron cell counts after chronic clozapine treatment were comparable to the control group. These results demonstrate that therapeutic concentrations of clozapine suppress nicotinic synaptic transmission in SCG cholinergic synapses, a simple in vitro preparation of cholinergic transmission.

Indexed as

AnimalsAntipsychotic AgentsCells, CulturedClozapineExcitatory Postsynaptic PotentialsMiceNeuronsSuperior Cervical GanglionSynaptic TransmissionAntipsychotic AgentsClozapineatypical antipsychotic drugscholinergic transmissionClozapinenicotinic receptorssEPSCssuperior cervical ganglion

Identifiers

PMID27627024
PMCPMC6061957
OpenAlexW2518968604

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.