ArticleOncotarget2016
Zanthoxylum fruit extract from Japanese pepper promotes autophagic cell death in cancer cells.
Article in Oncotarget, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Biotechnological Phytocomplex ofPharmaceutics · 2025Article
- Antitumor components and mechanisms ofFrontiers in pharmacology · 2025Review
- Dietary Replacement of Soybean Meal withAnimals : an open access journal from MDPI · 2024Article
- Hydroxy-γ-sanshool fromFrontiers in nutrition · 2022Article
- Elucidation of Japanese pepper (Zanthoxylum piperitum De Candolle) domestication using RAD-Seq.Scientific reports · 2021Article
- Costunolide and dehydrocostuslactone from Saussurea lappa root inhibit autophagy in hepatocellular carcinoma cells.Journal of natural medicines · 2021Article
- The inhibitory effect of TU-100 on hepatic stellate cell activation in the tumor microenvironment.Oncotarget · 2020Article
- Screening of Crude Drugs Used in Japanese Kampo Formulas for Autophagy-Mediated Cell Survival of the Human Hepatocellular Carcinoma Cell Line.Medicines (Basel, Switzerland) · 2019Article
- Medicinal Plants in the Prevention and Treatment of Colon Cancer.Oxidative medicine and cellular longevity · 2019Review
- Delphinidin induced protective autophagy via mTOR pathway suppression and AMPK pathway activation in HER-2 positive breast cancer cells.BMC cancer · 2018Article
- Depletion of gamma-glutamylcyclotransferase in cancer cells induces autophagy followed by cellular senescence.American journal of cancer research · 2018Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zanthoxylum fruit, obtained from the Japanese pepper plant (Zanthoxylum piperitum De Candolle), and its extract (Zanthoxylum fruit extract, ZFE) have multiple physiological activities (e.g., antiviral activity). However, the potential anticancer activity of ZFE has not been fully examined. In this study, we investigated the ability of ZFE to induce autophagic cell death (ACD). ZFE caused remarkable autophagy-like cytoplasmic vacuolization, inhibited cell proliferation, and ultimately induced cell death in the human cancer cell lines DLD-1, HepG2, and Caco-2, but not in A549, MCF-7, or WiDr cells. ZFE increased the level of LC3-II protein, a marker of autophagy. Knockdown of ATG5 using siRNA inhibited ZFE-induced cytoplasmic vacuolization and cell death. Moreover, in cancer cells that could be induced to undergo cell death by ZFE, the extract increased the phosphorylation of c-Jun N-terminal kinase (JNK), and the JNK inhibitor SP600125 attenuated both vacuolization and cell death. Based on morphology and expression of marker proteins, ZFE-induced cell death was neither apoptosis nor necrosis. Normal intestinal cells were not affected by ZFE. Taken together, our findings show that ZFE induces JNK-dependent ACD, which appears to be the main mechanism underlying its anticancer activity, suggesting a promising starting point for anticancer drug development.
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