ArticleMolecules (Basel, Switzerland)2016
Suitable DNA Barcoding for Identification and Supervision of Piper kadsura in Chinese Medicine Markets.
Article in Molecules (Basel, Switzerland), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed.
- The research of river basin ecological compensation based on water emissions trading mechanism.Water science and technology : a journal of the International Association on Water Pollution Research · 2024Article
- Galgravin Isolated fromInternational journal of molecular sciences · 2023Article
- Conventional and molecular pharmacognostic characters integrated with chemical profiles of five Piper plants in the Thai herbal pharmacopoeia and their admixture/adulteration/substitution situations in Thailand.Journal of natural medicines · 2022Article
- The specific DNA barcodes based on chloroplast genes for species identification ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Advancements and future prospective of DNA barcodes in the herbal drug industry.Frontiers in pharmacology · 2022Review
- DNA barcodes for the identification of Stephania (Menispermaceae) species.Molecular biology reports · 2020Article
- Evaluation of DNA markers for molecular identification of three Piper species from Brazilian Atlantic Rainforest.PloS one · 2020Article
- Integrated approach for identifying and evaluating the quality of Marsdenia tenacissima in the medicine market.PloS one · 2018Article
- Review
- Barcode ITS2: a useful tool for identifying Trachelospermum jasminoides and a good monitor for medicine market.Scientific reports · 2017Article
- Inspecting the True Identity of Herbal Materials fromFrontiers in plant science · 2017Article
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Authors and funding
10 authors.
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Abstract
Piper kadsura is a vine-like medicinal plant which is widely used in clinical treatment. However, P. kadsura is often substituted by other materials in the markets, thereby causing health risks. In this study, 38 P. kadsura samples and eight sequences from GenBank, including a closely-related species and common adulterants were collected. This study aimed to identify an effective DNA barcode from four popular DNA loci for P. kadsura authentication. The success rates of PCR amplification, sequencing, and sequence acquisition of matK were 10.5%, 75%, and 7.9%, respectively; for rbcL they were 89.5%, 8.8%, and 7.9%, respectively; ITS2 rates were 86.8%, 3.0%, and 2.6%, respectively, while for psbA-trnH they were all 100%, which is much higher than for the other three loci. The sequences were aligned using Muscle, genetic distances were computed using MEGA 5.2.2, and barcoding gap was performed using TAXON DNA. Phylogenetic analysis showed that psbA-trnH could clearly distinguish P. kadsura from its closely related species and the common adulterant. psbA-trnH was then used to evaluate the fake proportions of P. kadsura. Results showed that 18.4% of P. kadsura samples were fake, indicating that adulterant species exist in the Chinese markets. Two-dimensional DNA barcoding imaging of P. kadsura was conducted, which was beneficial to the management of P. kadsura. We conclude that the psbA-trnH region is a powerful tool for P. kadsura identification and supervision in the current medicine markets.
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