ArticleFrontiers in microbiology2016
Rapid and Sensitive Detection of Didymella bryoniae by Visual Loop-Mediated Isothermal Amplification Assay.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 40 citations in OpenAlex.
- Advancing fungal phylogenetics: integrating modern sequencing, dark taxa discovery, and machine learning.Archives of microbiology · 2025Review
- A comprehensive guide to loop-mediated isothermal amplification, an emerging diagnostic tool for plant pathogenic fungi.Frontiers in plant science · 2025Review
- LAMP Reaction in Plant Disease Surveillance: Applications, Challenges, and Future Perspectives.Life (Basel, Switzerland) · 2024Review
- Advances in the detection technology of vegetable soil borne fungi and bacteria.Frontiers in microbiology · 2024Review
- Gummy stem blight: One disease, three pathogens.Molecular plant pathology · 2023Review
- Isothermal nucleic acid amplification and its uses in modern diagnostic technologies.3 Biotech · 2023Review
- Mycobiomes of the Ocular Surface in Bacterial Keratitis Patients.Frontiers in ophthalmology · 2022Article
- Antifungal Activity and Chemical Composition of Seven Essential Oils to Control the Main Seedborne Fungi of Cucurbits.Antibiotics (Basel, Switzerland) · 2021Article
- Detection and Quantification ofFrontiers in microbiology · 2021Article
- A Powerful LAMP Weapon against the Threat of the Quarantine Plant PathogenMicroorganisms · 2020Article
- Genome-scale analyses and characteristics of putative pathogenicity genes of Stagonosporopsis cucurbitacearum, a pumpkin gummy stem blight fungus.Scientific reports · 2020Article
- Recent Advances in Molecular Diagnostics of Fungal Plant Pathogens: A Mini Review.Frontiers in cellular and infection microbiology · 2020Review
- Metagenomics Reveal Correlations Between Microbial Organisms in Soils and the Health ofFrontiers in microbiology · 2020Article
- GLAPD: Whole Genome Based LAMP Primer Design for a Set of Target Genomes.Frontiers in microbiology · 2019Article
- Development and evaluation of LAMP, CPA and IMSA methods for rapid detection of theExperimental and therapeutic medicine · 2018Article
- Development and Evaluation of a Loop-Mediated Isothermal Amplification Assay for the Detection ofThe American journal of tropical medicine and hygiene · 2017Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Didymella bryoniae is a pathogenic fungus that causes gummy stem blight (GSB) in Cucurbitaceae crops (e.g., cantaloupe, muskmelon, cucumber, and watermelon). GSB produces lesions on the stems and leaves, and can also be spread by seeds. Here, we developed a rapid, visual, and sensitive loop-mediated amplification (LAMP) assay for D. bryoniae detection based on sequence-characterized amplified regions (GenBank accession nos GQ872461 and GQ872462) common to the two random amplification of polymorphic DNA group genotypes (RGI and RGII) of D. bryoniae; ideal conditions for detection were optimized for completion in 45 min at 63°C. The sensitivity and specificity of the LAMP assay were further analyzed in comparison with those of a conventional polymerase chain reaction (PCR). The sensitivity of the LAMP assay was 1000-fold higher than that of conventional PCR with a detection limit of 0.1 fg μL(-1) of targeted DNA. The LAMP assay could be accomplished in about 45 min, with the results visible to the naked eye. The assay showed high specificity in discriminating all D. bryoniae isolates from seven other fungal pathogens that occur in Cucurbitaceae crops. The LAMP assay also detected D. bryoniae infection in young muskmelon leaves with suspected early symptoms of GSB disease. Hence, the technique has great potential for developing rapid and sensitive visual detection methods for the D. bryoniae pathogen in crops and seeds. This method has potential application in early prediction of disease and reducing the risk of epidemics.
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