ArticleFrontiers in microbiology2016
Construction of a Multiplex Promoter Reporter Platform to Monitor Staphylococcus aureus Virulence Gene Expression and the Identification of Usnic Acid as a Potent Suppressor of psm Gene Expression.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 13 citations in OpenAlex.
- Synthesis and Biological Evaluation of Novel C-28 Chloroacetamide-Modified Ursolic Acid Derivatives as Antibacterial Agents.Microorganisms · 2026Article
- Toolbox of Characterized Genetic Parts forACS synthetic biology · 2024Article
- SaeR as a novel target for antivirulence therapy againstEmerging microbes & infections · 2023Article
- Identification of a Small Molecule Compound Active against Antibiotic-TolerantInternational journal of molecular sciences · 2023Article
- Regulation ofPharmaceutics · 2023Review
- Subinhibitory Concentrations of Antibiotics Exacerbate Staphylococcal Infection by Inducing Bacterial Virulence.Microbiology spectrum · 2022Article
- Antivirulence Agent as an Adjuvant of β-Lactam Antibiotics in Treating Staphylococcal Infections.Antibiotics (Basel, Switzerland) · 2022Article
- Staphylococcal species less frequently isolated from human clinical specimens - are they a threat for hospital patients?BMC infectious diseases · 2020Article
- Suppression ofProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Growth-dependent activity of the cold shock cspA promoter + 5' UTR and production of the protein CspA in Staphylococcus aureus Newman.BMC research notes · 2017Article
- CcpA Affects Infectivity ofFrontiers in cellular and infection microbiology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As antibiotic resistance becomes phenomenal, alternative therapeutic strategies for bacterial infections such as anti-virulence treatments have been advocated. We have constructed a total of 20 gfp-luxABCDE dual-reporter plasmids with selected promoters from S. aureus virulence-associated genes. The plasmids were introduced into various S. aureus strains to establish a gfp-lux based multiplex promoter reporter platform for monitoring S. aureus virulence gene expressions in real time to identify factors or compounds that may perturb virulence of S. aureus. The gene expression profiles monitored by luminescence correlated well with qRT-PCR results and extrinsic factors including carbon dioxide and some antibiotics were shown to suppress or induce the expression of virulence factors in this platform. Using this platform, sub-inhibitory ampicillin was shown to be a potent inducer for the expression of many virulence factors in S. aureus. Bacterial adherence and invasion assays using mammalian cells were employed to measure S. aureus virulence induced by ampicillin. The platform was used for screening of natural extracts that perturb the virulence of S. aureus and usnic acid was identified to be a potent repressor for the expression of psm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.