ArticleFrontiers in microbiology2016
Metagenomic and PCR-Based Diversity Surveys of [FeFe]-Hydrogenases Combined with Isolation of Alkaliphilic Hydrogen-Producing Bacteria from the Serpentinite-Hosted Prony Hydrothermal Field, New Caledonia.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 22 citations in OpenAlex.
- Detection of Methicillin-resistant Staphylococcus aureus (MRSA) and biofilm formation among dental patients and dental health care workers: cross sectional study.Clinical oral investigations · 2026Article
- Cultivating microbial communities from the serpentinite-hosted Prony Bay hydrothermal field on different carbon sources in hydrogen-fed bioreactors.Environmental microbiome · 2025Article
- Microbial ecology of serpentinite-hosted ecosystems.The ISME journal · 2025Review
- A non-methanogenic archaeon within the order Methanocellales.Nature communications · 2024Article
- Microbial taxa related to natural hydrogen and methane emissions in serpentinite-hosted hyperalkaline springs of New Caledonia.Frontiers in microbiology · 2023Article
- Metabolic challenges and key players in serpentinite-hosted microbial ecosystems.Frontiers in microbiology · 2023Review
- Article
- Procaryotic Diversity and Hydrogenotrophic Methanogenesis in an Alkaline Spring (La Crouen, New Caledonia).Microorganisms · 2021Article
- Formation and loss of metastable brucite: does Fe(II)-bearing brucite support microbial activity in serpentinizing ecosystems?Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2020Article
- Influence of Hydrogen Electron Donor, Alkaline pH, and High Nitrate Concentrations on Microbial Denitrification: A Review.International journal of molecular sciences · 2019Review
- Molecular Hydrogen, a Neglected Key Driver of Soil Biogeochemical Processes.Applied and environmental microbiology · 2019Review
- Diversity of Rare and Abundant Prokaryotic Phylotypes in the Prony Hydrothermal Field and Comparison with Other Serpentinite-Hosted Ecosystems.Frontiers in microbiology · 2018Article
- Genomic andFrontiers in microbiology · 2018Article
- Geological and Geochemical Controls on Subsurface Microbial Life in the Samail Ophiolite, Oman.Frontiers in microbiology · 2017Article
- Evaluating Serpentinization as a Source of Phosphite to Microbial Communities in Hydrothermal Vents.GeobiologyArticle
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10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High amounts of hydrogen are emitted in the serpentinite-hosted hydrothermal field of the Prony Bay (PHF, New Caledonia), where high-pH (~11), low-temperature (< 40°C), and low-salinity fluids are discharged in both intertidal and shallow submarine environments. In this study, we investigated the diversity and distribution of potentially hydrogen-producing bacteria in Prony hyperalkaline springs by using metagenomic analyses and different PCR-amplified DNA sequencing methods. The retrieved sequences of hydA genes, encoding the catalytic subunit of [FeFe]-hydrogenases and, used as a molecular marker of hydrogen-producing bacteria, were mainly related to those of Firmicutes and clustered into two distinct groups depending on sampling locations. Intertidal samples were dominated by new hydA sequences related to uncultured Firmicutes retrieved from paddy soils, while submarine samples were dominated by diverse hydA sequences affiliated with anaerobic and/or thermophilic submarine Firmicutes pertaining to the orders Thermoanaerobacterales or Clostridiales. The novelty and diversity of these [FeFe]-hydrogenases may reflect the unique environmental conditions prevailing in the PHF (i.e., high-pH, low-salt, mesothermic fluids). In addition, novel alkaliphilic hydrogen-producing Firmicutes (Clostridiales and Bacillales) were successfully isolated from both intertidal and submarine PHF chimney samples. Both molecular and cultivation-based data demonstrated the ability of Firmicutes originating from serpentinite-hosted environments to produce hydrogen by fermentation, potentially contributing to the molecular hydrogen balance in situ.
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