ArticleBlood2016
Respective contributions of single and compound granule fusion to secretion by activated platelets.
Article in Blood, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 70 citations in OpenAlex.
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- Nanoscale insights into hematology: super-resolved imaging on blood cell structure, function, and pathology.Journal of nanobiotechnology · 2024Review
- Platelet Activation Pathways Controlling Reversible Integrin αIIbβ3 Activation.TH open : companion journal to thrombosis and haemostasis · 2024Article
- Platelet C3G: a key player in vesicle exocytosis, spreading and clot retraction.Cellular and molecular life sciences : CMLS · 2024Article
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- The fate of mitochondria during platelet activation.Blood advances · 2023Article
- Lenalidomide Promotes Thrombosis Formation, but Does Not Affect Platelet Activation in Multiple Myeloma.International journal of molecular sciences · 2023Article
- Preeclampsia: Platelet procoagulant membrane dynamics and critical biomarkers.Research and practice in thrombosis and haemostasis · 2023Article
- ADP receptor P2Y12 is the capstone of the cross-talk between CaResearch and practice in thrombosis and haemostasis · 2023Article
- Targeting Platelet Activation Pathways to Limit Tumour Progression: Current State of Affairs.Pharmaceuticals (Basel, Switzerland) · 2022Review
- Effects of Platelet Agonists and Priming on the Formation of Platelet Populations.Thrombosis and haemostasis · 2022Article
- Mechanisms Underlying Dichotomous Procoagulant COAT Platelet Generation-A Conceptual Review Summarizing Current Knowledge.International journal of molecular sciences · 2022Review
- Quantitative 3D microscopy highlights altered von Willebrand factor α-granule storage in patients with von Willebrand disease with distinct pathogenic mechanisms.Research and practice in thrombosis and haemostasis · 2021Article
- Structural analysis of resting mouse platelets by 3D-EM reveals an unexpected variation in α-granule shape.Platelets · 2021Article
- Sanguinarine Attenuates Collagen-Induced Platelet Activation and Thrombus Formation.Biomedicines · 2021Article
- Platelet Phenotyping and Function Testing in Thrombocytopenia.Journal of clinical medicine · 2021Review
- Canalicular system reorganization during mouse platelet activation as revealed by 3D ultrastructural analysis.Platelets · 2021Article
- Platelet δ-Storage Pool Disease: An Update.Journal of clinical medicine · 2020Review
- Use of electron microscopy to study platelets and thrombi.Platelets · 2020Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Although granule secretion is pivotal in many platelet responses, the fusion routes of α and δ granule release remain uncertain. We used a 3D reconstruction approach based on electron microscopy to visualize the spatial organization of granules in unstimulated and activated platelets. Two modes of exocytosis were identified: a single mode that leads to release of the contents of individual granules and a compound mode that leads to the formation of granule-to-granule fusion, resulting in the formation of large multigranular compartments. Both modes occur during the course of platelet secretion. Single fusion events are more visible at lower levels of stimulation and early time points, whereas large multigranular compartments are present at higher levels of agonist and at later time points. Although α granules released their contents through both modes of exocytosis, δ granules underwent only single exocytosis. To define the underlying molecular mechanisms, we examined platelets from vesicle-associated membrane protein 8 (VAMP8) null mice. After weak stimulation, compound exocytosis was abolished and single exocytosis decreased in VAMP8 null platelets. Higher concentrations of thrombin bypassed the VAMP8 requirement, indicating that this isoform is a key but not a required factor for single and/or compound exocytosis. Concerning the biological relevance of our findings, compound exocytosis was observed in thrombi formed after severe laser injury of the vessel wall with thrombin generation. After superficial injury without thrombin generation, no multigranular compartments were detected. Our studies suggest that platelets use both modes of membrane fusion to control the extent of agonist-induced exocytosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.