ArticleMolecular & cellular proteomics : MCP2016
Molecular Profiling of Phagocytic Immune Cells in Anopheles gambiae Reveals Integral Roles for Hemocytes in Mosquito Innate Immunity.
Article in Molecular & cellular proteomics : MCP, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 65 citations in OpenAlex.
- Article
- The Relative Contributions ofInsects · 2026Article
- Blood feeding, microbiota, and layered mosquito immunity: from pathway phenotypes to vector competence.Frontiers in immunology · 2026Review
- Nymphal diets boost adults' immunity via strengthened constitutive immunity and metabolic capacity in Adelphocoris suturalis.Communications biology · 2025Article
- Characterization of Anopheles gambiae immune cells through genetic and functional immunophenotyping.Nature communications · 2025Article
- A genome-wide CRISPR screen in Anopheles mosquito cells identifies fitness and immune cell function-related genes.Nature communications · 2025Article
- Article
- Dietary L-3,4-dihydroxyphenylalanine (L-DOPA) augments cuticular melanization in Anopheles mosquitos reducing their lifespan and malaria burden.Nature communications · 2025Article
- Mosquito immune cells enhance dengue and Zika virus infection in Aedes aegypti.Nature communications · 2025Article
- TNF signaling mediates cellular immune function and promotes malaria parasite killing in the mosquito Anopheles gambiae.PLoS pathogens · 2025Article
- Protocol to chemically deplete phagocytic hemocytes in Anopheles gambiae using clodronate liposomes.STAR protocols · 2025Article
- Influence of blood feeding and infection on arthropod hemocytes.Current opinion in insect science · 2025Review
- Advances in the dissection of Anopheles-Plasmodium interactions.PLoS pathogens · 2025Review
- Anopheles gambiae phagocytic hemocytes promote Plasmodium falciparum infection by regulating midgut epithelial integrity.Nature communications · 2025Article
- Innate Immunity in Insects: The Lights and Shadows of Phenoloxidase System Activation.International journal of molecular sciences · 2025Review
- Croquemort-scavenger receptor class B 2 (Journal of biosciences · 2025Article
- Azadirachtin disrupts ecdysone signaling and alters sand fly immunity.Parasites & vectors · 2024Article
- Exploring new dimensions of immune cell biology inbioRxiv : the preprint server for biology · 2024Article
- Mitochondrial Variation in Anopheles gambiae and Anopheles coluzzii: Phylogeographic Legacy and Mitonuclear Associations With Metabolic Resistance to Pathogens and Insecticides.Genome biology and evolution · 2024Article
- Article
Corrections and comments
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Authors and funding
7 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The innate immune response is highly conserved across all eukaryotes and has been studied in great detail in several model organisms. Hemocytes, the primary immune cell population in mosquitoes, are important components of the mosquito innate immune response, yet critical aspects of their biology have remained uncharacterized. Using a novel method of enrichment, we isolated phagocytic granulocytes and quantified their proteomes by mass spectrometry. The data demonstrate that phagocytosis, blood-feeding, and Plasmodium falciparum infection promote dramatic shifts in the proteomic profiles of An. gambiae granulocyte populations. Of interest, large numbers of immune proteins were induced in response to blood feeding alone, suggesting that granulocytes have an integral role in priming the mosquito immune system for pathogen challenge. In addition, we identify several granulocyte proteins with putative roles as membrane receptors, cell signaling, or immune components that when silenced, have either positive or negative effects on malaria parasite survival. Integrating existing hemocyte transcriptional profiles, we also compare differences in hemocyte transcript and protein expression to provide new insight into hemocyte gene regulation and discuss the potential that post-transcriptional regulation may be an important component of hemocyte gene expression. These data represent a significant advancement in mosquito hemocyte biology, providing the first comprehensive proteomic profiling of mosquito phagocytic granulocytes during homeostasis blood-feeding, and pathogen challenge. Together, these findings extend current knowledge to further illustrate the importance of hemocytes in shaping mosquito innate immunity and their principal role in defining malaria parasite survival in the mosquito host.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.