ArticleScientific reports2016
The Crystal Structure of Peroxiredoxin Asp f3 Provides Mechanistic Insight into Oxidative Stress Resistance and Virulence of Aspergillus fumigatus.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- ZipV Is Required for Oxidative Stress Resistance and Pathogenicity inJournal of fungi (Basel, Switzerland) · 2026Article
- Aspergillus fumigatus dsRNA virus promotes fungal fitness and pathogenicity in the mammalian host.Nature microbiology · 2025Article
- Residential exposure toThe European respiratory journal · 2024Article
- New developments in Aspergillus fumigatus and host reactive oxygen species responses.Current opinion in microbiology · 2024Review
- The Oxidative Stress Response Highly Depends on Glucose and Iron Availability inJournal of fungi (Basel, Switzerland) · 2024Article
- AoPrdx2 Regulates Oxidative Stress, Reactive Oxygen Species, Trap Formation, and Secondary Metabolism inJournal of fungi (Basel, Switzerland) · 2024Article
- Cytosol Peroxiredoxin and Cell Surface Catalase Differentially Respond to HAntioxidants (Basel, Switzerland) · 2023Article
- Comparative proteome analysis identifies species-specific signature proteins in Aspergillus pathogens.Applied microbiology and biotechnology · 2023Article
- Oxidative stress response pathways in fungi.Cellular and molecular life sciences : CMLS · 2022Review
- Sulfur Metabolism as a Promising Source of New Antifungal Targets.Journal of fungi (Basel, Switzerland) · 2022Review
- Article
- Increasing NADPH impairs fungal HBioresources and bioprocessing · 2022Article
- Article
- Relevance of peroxiredoxins in pathogenic microorganisms.Applied microbiology and biotechnology · 2021Review
- Fungal and host protein persulfidation are functionally correlated and modulate both virulence and antifungal response.PLoS biology · 2021Article
- The Peroxiredoxin Asp f3 Acts as Redox Sensor inGenes · 2021Article
- Structure-guided approaches to targeting stress responses in human fungal pathogens.The Journal of biological chemistry · 2020Review
- Biotinylated Surfome Profiling Identifies Potential Biomarkers for Diagnosis and Therapy of Aspergillus fumigatus Infection.mSphere · 2020Article
- The Pheromone Module SteC-MkkB-MpkB-SteD-HamE Regulates Development, Stress Responses and Secondary Metabolism inFrontiers in microbiology · 2020Article
- The Essential Thioredoxin Reductase of the Human Pathogenic MoldFrontiers in microbiology · 2020Article
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Authors and funding
9 authors.
Funding
Abstract
Invasive aspergillosis and other fungal infections occur in immunocompromised individuals, including patients who received blood-building stem cell transplants, patients with chronic granulomatous disease (CGD), and others. Production of reactive oxygen species (ROS) by immune cells, which incidentally is defective in CGD patients, is considered to be a fundamental process in inflammation and antifungal immune response. Here we show that the peroxiredoxin Asp f3 of Aspergillus fumigatus inactivates ROS. We report the crystal structure and the catalytic mechanism of Asp f3, a two-cysteine type peroxiredoxin. The latter exhibits a thioredoxin fold and a homodimeric structure with two intermolecular disulfide bonds in its oxidized state. Replacement of the Asp f3 cysteines with serine residues retained its dimeric structure, but diminished Asp f3's peroxidase activity, and extended the alpha-helix with the former peroxidatic cysteine residue C61 by six residues. The asp f3 deletion mutant was sensitive to ROS, and this phenotype was rescued by ectopic expression of Asp f3. Furthermore, we showed that deletion of asp f3 rendered A. fumigatus avirulent in a mouse model of pulmonary aspergillosis. The conserved expression of Asp f3 homologs in medically relevant molds and yeasts prompts future evaluation of Asp f3 as a potential therapeutic target.
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