ArticleScientific reports2016
Efficient Production of Fluorescent Transgenic Rats using the piggyBac Transposon.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Highly efficient and low-mosaicismiScience · 2026Article
- Article
- Article
- Stable long-term germline transmission of GFP transgenic rat via PiggyBac transposon mediated gene transfer.BMC veterinary research · 2024Article
- miR-30e-5p attenuates neuronal deficit and inflammation of rats with intracerebral hemorrhage by regulating TLR4.Experimental and therapeutic medicine · 2022Article
- Application of transposon systems in the transgenesis of bovine somatic and germ cells.BMC veterinary research · 2022Article
- Genome-scale screening in a rat haploid system identifies Thop1 as a modulator of pluripotency exit.Cell proliferation · 2022Article
- Contemporary Transposon Tools: A Review and Guide through Mechanisms and Applications ofInternational journal of molecular sciences · 2021Review
- Applications ofStem cells international · 2021Review
- Review
- In VivoInternational journal of molecular sciences · 2019Article
- Busting the myth: more good than harm in transgenic cells.Neural regeneration research · 2019Article
- Long-term health and germline transmission in transgenic cattle following transposon-mediated gene transfer.BMC genomics · 2018Article
- Development of genome engineering technologies in cattle: from random to specific.Journal of animal science and biotechnology · 2018Review
- Transgenic Mouse Models in Cancer Research.Frontiers in oncology · 2018Review
- From engineering to editing the rat genome.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rats with fluorescent markers are of great value for studies that trace lineage-specific development, particularly those assessing the differentiation potential of embryonic stem cells (ESCs). The piggyBac (PB) transposon is widely used for the efficient introduction of genetic modifications into genomes, and has already been successfully used to produce transgenic mice and rats. Here, we generated transgenic rats carrying either the desRed fluorescent protein (RFP) gene or the enhanced green fluorescent protein (eGFP) gene by injecting pronuclei with PB plasmids. We showed that the transgenic rats expressed the RFP or eGFP gene in many organs and had the capability to transmit the marker gene to the next generation through germline integration. In addition, rat embryonic stem cells (ESCs) carrying an RFP reporter gene can be derived from the blastocysts of the transgenic rats. Moreover, the RFP gene can be detected in chimeras derived from RFP ESCs via blastocyst injection. This work suggests that PB-mediated transgenesis is a powerful tool to generate transgenic rats expressing fluorescent proteins with high efficiency, and this technique can be used to derive rat ESCs expressing a reporter protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.