ArticleProceedings of the National Academy of Sciences of the United States of America1989
Actin filaments mediate Dictyostelium myosin assembly in vitro.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
- Limiting pool and actin architecture controls myosin cluster sizes in adherent cells.Biophysical journal · 2024Article
- Filament evanescence of myosin II and smooth muscle function.The Journal of general physiology · 2021Review
- How the mechanobiome drives cell behavior, viewed through the lens of control theory.Journal of cell science · 2019Review
- Myosin Crossbridge, Contractile Unit, and the Mechanism of Contraction in Airway Smooth Muscle: A Mechanical Engineer's Perspective.Journal of engineering and science in medical diagnostics and therapy · 2019Review
- Actin dynamics and competition for myosin monomer govern the sequential amplification of myosin filaments.Nature cell biology · 2017Article
- Cytokinesis: Robust cell shape regulation.Seminars in cell & developmental biology · 2016Review
- Cell shape regulation through mechanosensory feedback control.Journal of the Royal Society, Interface · 2015Article
- Kinetic Monte Carlo simulations of the assembly of filamentous biomacromolecules by dimer addition mechanism.RSC advances · 2015Article
- Genetic suppression of a phosphomimic myosin II identifies system-level factors that promote myosin II cleavage furrow accumulation.Molecular biology of the cell · 2014Article
- Myosin filaments in smooth muscle cells do not have a constant length.The Journal of physiology · 2013Article
- A mechanosensory system governs myosin II accumulation in dividing cells.Molecular biology of the cell · 2012Article
- Understanding the cooperative interaction between myosin II and actin cross-linkers mediated by actin filaments during mechanosensation.Biophysical journal · 2012Article
- Cytotoxic Effects on HL-60 Cells of Myosin Light Chain Kinase Inhibitor ML-7 Alone and in Combination with Flavonoids.Toxicological research · 2009Article
- Disrupting actin-myosin-actin connectivity in airway smooth muscle as a treatment for asthma?Proceedings of the American Thoracic Society · 2009Review
- Nonmuscle myosin II is responsible for maintaining endothelial cell basal tone and stress fiber integrity.American journal of physiology. Cell physiology · 2008Article
- Assembly of transverse tubule architecture in the middle and myotendinous junctional regions in developing rat skeletal muscle fibers.Journal of muscle research and cell motility · 2007Article
- Virulence and functions of myosin II are inhibited by overexpression of light meromyosin in Entamoeba histolytica.Molecular biology of the cell · 1998Article
- A fluorescent protein biosensor of myosin II regulatory light chain phosphorylation reports a gradient of phosphorylated myosin II in migrating cells.Molecular biology of the cell · 1995Article
- Myosin light chain kinase-regulated endothelial cell contraction: the relationship between isometric tension, actin polymerization, and myosin phosphorylation.The Journal of cell biology · 1995Article
- Release of myosin II from the membrane-cytoskeleton of Dictyostelium discoideum mediated by heavy-chain phosphorylation at the foci within the cortical actin network.The Journal of cell biology · 1992Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Because myosin thick filaments form in the actin-rich cortex of nonmuscle cells, we have examined the role of Dictyostelium actin filaments in the assembly of Dictyostelium myosin (type II). Fluorescence energy transfer and light-scattering assembly assays indicate that self-association of Dictyostelium myosin into bipolar thick filaments is kinetically regulated by actin filament networks. Regulation is nucleotide dependent but does not require ATP hydrolysis. Myosin assembly is accelerated approximately 5-fold by actin filaments when either 1 mM ATP or 1 mM adenosine 5'-[beta,gamma-imido]triphosphate (AMP-P[NH]P) is present. However, actin filaments together with 1 mM ADP abolish myosin assembly. Accelerated assembly appears to require transient binding of myosin molecules to actin filaments before incorporation into thick filaments. Fluorescence energy-transfer assays demonstrate that myosin associates with actin filaments at a rate that is equivalent to the accelerated myosin assembly rate, evidence that myosin to actin binding is a rate-limiting step in accelerated thick filament formation. Actin filament networks are also implicated in regulation of thick filament formation, since fragmentation of F-actin networks by severin causes immediate cessation of accelerated myosin assembly. Electron microscopic studies support a model of actin filament-mediated myosin assembly. In ADP, myosin monomers rapidly decorate F-actin, preventing extensive formation of thick filaments. In AMP-P[NH]P, myosin assembles along actin filaments, forming structures that resemble primitive stress fibers. Taken together, these data suggest a model in which site-directed assembly of thick filaments in Dictyostelium is mediated by the interaction of myosin monomers with cortical actin filament networks.
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