ArticleProceedings of the National Academy of Sciences of the United States of America1989
Isolation and characterization of cDNA encoding the alpha subunit of Cap Z(36/32), an actin-capping protein from the Z line of skeletal muscle.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 47 citations in OpenAlex.
- Sarcomere maturation: function acquisition, molecular mechanism, and interplay with other organelles.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Article
- PIM1 accelerates prostate cancer cell motility by phosphorylating actin capping proteins.Cell communication and signaling : CCS · 2020Article
- Muscle Contraction.Cold Spring Harbor perspectives in biology · 2018Review
- Prognostic value of CAPZA1 overexpression in gastric cancer.International journal of oncology · 2013Article
- Identification of novel citrullinated autoantigens of synovium in rheumatoid arthritis using a proteomic approach.Arthritis research & therapy · 2006Article
- Generation of functional beta-actinin (CapZ) in an E. coli expression system.Journal of muscle research and cell motility · 1998Article
- Molecular cloning, expression, and mapping of the high affinity actin-capping domain of chicken cardiac tensin.The Journal of cell biology · 1995Article
- Inhibition of CapZ during myofibrillogenesis alters assembly of actin filaments.The Journal of cell biology · 1995Article
- Ultrastructural analysis of the dynactin complex: an actin-related protein is a component of a filament that resembles F-actin.The Journal of cell biology · 1994Article
- The alpha and beta subunits of nematode actin capping protein function in yeast.Molecular biology of the cell · 1993Article
- Effects of null mutations and overexpression of capping protein on morphogenesis, actin distribution and polarized secretion in yeast.The Journal of cell biology · 1992Article
- Karyoplasmic interaction selection strategy: a general strategy to detect protein-protein interactions in mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Identification and characterization of an actin-binding site of CapZ.The Journal of cell biology · 1992Article
- Radixin is a novel member of the band 4.1 family.The Journal of cell biology · 1991Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
cDNA encoding the alpha chain of Cap Z has been isolated by screening a lambda gt11 library with affinity-purified antibodies. A single cDNA insert (designated CE2) of 2153 base pairs (bp) contains an open reading frame of 836 bp, which is incomplete at its 5' end. The technique of "rapid amplification of cDNA ends" has been used to extend the 5' end of this open reading frame to a potential transcription initiation site that is preceded by 320 bp of an apparently untranslated region. The protein predicted by the resulting nucleotide sequence has a Mr of 32,960 and contains four regions that show close homology with four alpha-chymotryptic digestion fragments of the alpha chain. The amino acid composition of the alpha chain of Cap Z and the predicted protein are also similar. Northern blot analysis of whole chicken embryos shows two mRNA species of 1.9 and 2.4 kilobases, respectively, that hybridize with CE2. Three potential polyadenylylation signals in two regions of CE2 460 bp apart are identified, suggesting that the two messages may result from the use of alternative polyadenylylation sites. Comparison of the sequence data with that of other known actin-capping and severing proteins shows no significant homologies, suggesting that Cap Z may be a member of a unique group of capping, nonsevering proteins.
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