ArticleNano letters2016
Magnetically Actuated Protease Sensors for in Vivo Tumor Profiling.
Article in Nano letters, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 51 citations in OpenAlex.
- Renal clearable CRISPR nanosensor targeting mitochondrial DNA mutation for noninvasive monitoring of tumor progression and metastasis.Science advances · 2025Article
- Synthetic Protein-to-DNA Input Exchange for Protease Activity Detection Using CRISPR-Cas12a.Analytical chemistry · 2024Article
- Review
- Switch-on Fluorescence Analysis of Protease Activity with the Assistance of a Nickel Ion-Nitrilotriacetic Acid-Conjugated Magnetic Nanoparticle.Molecules (Basel, Switzerland) · 2023Article
- Modulating cell signalling in vivo with magnetic nanotransducers.Nature reviews. Methods primers · 2022Article
- The Tumor Proteolytic Landscape: A Challenging Frontier in Cancer Diagnosis and Therapy.International journal of molecular sciences · 2021Review
- Magnetothermal Multiplexing for Selective Remote Control of Cell Signaling.Advanced functional materials · 2020Article
- Engineering synthetic breath biomarkers for respiratory disease.Nature nanotechnology · 2020Article
- Transgene-free remote magnetothermal regulation of adrenal hormones.Science advances · 2020Article
- Towards extracellular matrix normalization for improved treatment of solid tumors.Theranostics · 2020Review
- Remotely controlled chemomagnetic modulation of targeted neural circuits.Nature nanotechnology · 2019Article
- Deconvolving multiplexed protease signatures with substrate reduction and activity clustering.PLoS computational biology · 2019Article
- Renal clearable catalytic gold nanoclusters for in vivo disease monitoring.Nature nanotechnology · 2019Article
- Theoretical Analysis for Wireless Magnetothermal Deep Brain Stimulation Using Commercial Nanoparticles.International journal of molecular sciences · 2019Article
- Thermo-Sensitive Nanomaterials: Recent Advance in Synthesis and Biomedical Applications.Nanomaterials (Basel, Switzerland) · 2018Review
- Functional profiling of circulating tumor cells with an integrated vortex capture and single-cell protease activity assay.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Probing Cellular Processes Using Engineered Nanoparticles.Bioconjugate chemistry · 2018Review
- Practical methods for generating alternating magnetic fields for biomedical research.The Review of scientific instruments · 2017Article
- Theranostic Probes for Targeting Tumor Microenvironment: An Overview.International journal of molecular sciences · 2017Review
- In Vivo Biosensing: Progress and Perspectives.ACS sensors · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Targeted cancer therapies require a precise determination of the underlying biological processes driving tumorigenesis within the complex tumor microenvironment. Therefore, new diagnostic tools that capture the molecular activity at the disease site in vivo are needed to better understand tumor behavior and ultimately maximize therapeutic responses. Matrix metalloproteinases (MMPs) drive multiple aspects of tumorigenesis, and their activity can be monitored using engineered peptide substrates as protease-specific probes. To identify tumor specific activity profiles, local sampling of the tumor microenvironment is necessary, such as through remote control of probes, which are only activated at the tumor site. Alternating magnetic fields (AMFs) provide an attractive option to remotely apply local triggering signals because they penetrate deep into the body and are not likely to interfere with biological processes due to the weak magnetic properties of tissue. Here, we report the design and evaluation of a protease-activity nanosensor that can be remotely activated at the site of disease via an AMF at 515 kHz and 15 kA/m. Our nanosensor was composed of thermosensitive liposomes containing functionalized protease substrates that were unveiled at the target site by remotely triggered heat dissipation of coencapsulated magnetic nanoparticles (MNPs). This nanosensor was combined with a unique detection assay to quantify the amount of cleaved substrates in the urine. We applied this spatiotemporally controlled system to determine tumor protease activity in vivo and identified differences in substrate cleavage profiles between two mouse models of human colorectal cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.