ArticleMolecular plant2016
Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components.
Article in Molecular plant, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Over 25 years of decrypting PIN-mediated plant development.Nature communications · 2024Review
- Mapping the membrane orientation of auxin homeostasis regulators PIN5 and PIN8 in Arabidopsis thaliana root cells reveals their divergent topology.Plant methods · 2024Article
- Role of transcriptional regulation in auxin-mediated response to abiotic stresses.Frontiers in genetics · 2024Review
- Auxin and abiotic stress responses.Journal of experimental botany · 2023Review
- Substrate recognition and transport mechanism of the PIN-FORMED auxin exporters.Trends in biochemical sciences · 2023Review
- Characterization of the PIN Auxin Efflux Carrier Gene Family and Its Expression during Zygotic Embryogenesis inPlants (Basel, Switzerland) · 2023Article
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- The Hydrophilic Loop ofInternational journal of molecular sciences · 2022Article
- Auxin Transporters-A Biochemical View.Cold Spring Harbor perspectives in biology · 2022Review
- Characterization and expression profiling of PIN auxin efflux transporters reveal their role in developmental and abiotic stress conditions in rice.Frontiers in plant science · 2022Article
- EuryaleFrontiers in plant science · 2022Article
- The evolutionary history of topological variations in the CPA/AT transporters.PLoS computational biology · 2021Article
- ER-Localized PIN Carriers: Regulators of Intracellular Auxin Homeostasis.Plants (Basel, Switzerland) · 2020Review
- Multi-Omics Strategies for Decoding Smoke-Assisted Germination Pathways and Seed Vigour.International journal of molecular sciences · 2020Review
- Directional auxin fluxes in plants by intramolecular domain-domain coevolution of PIN auxin transporters.The New phytologist · 2020Article
- The Nuts and Bolts of PIN Auxin Efflux Carriers.Frontiers in plant science · 2019Review
- The Inhibitor Endosidin 4 Targets SEC7 Domain-Type ARF GTPase Exchange Factors and Interferes with Subcellular Trafficking in Eukaryotes.The Plant cell · 2018Article
- The PIN-FORMED Auxin Efflux Carriers in Plants.International journal of molecular sciences · 2018Review
- A Functional Study of AUXILIN-LIKE1 and 2, Two Putative Clathrin Uncoating Factors in Arabidopsis.The Plant cell · 2018Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Auxin directs plant ontogenesis via differential accumulation within tissues depending largely on the activity of PIN proteins that mediate auxin efflux from cells and its directional cell-to-cell transport. Regardless of the developmental importance of PINs, the structure of these transporters is poorly characterized. Here, we present experimental data concerning protein topology of plasma membrane-localized PINs. Utilizing approaches based on pH-dependent quenching of fluorescent reporters combined with immunolocalization techniques, we mapped the membrane topology of PINs and further cross-validated our results using available topology modeling software. We delineated the topology of PIN1 with two transmembrane (TM) bundles of five α-helices linked by a large intracellular loop and a C-terminus positioned outside the cytoplasm. Using constraints derived from our experimental data, we also provide an updated position of helical regions generating a verisimilitude model of PIN1. Since the canonical long PINs show a high degree of conservation in TM domains and auxin transport capacity has been demonstrated for Arabidopsis representatives of this group, this empirically enhanced topological model of PIN1 will be an important starting point for further studies on PIN structure-function relationships. In addition, we have established protocols that can be used to probe the topology of other plasma membrane proteins in plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.