ArticleCurrent protocols in chemical biology2016
Fabrication of 3-D Reconstituted Organoid Arrays by DNA-Programmed Assembly of Cells (DPAC).
Article in Current protocols in chemical biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- A universal surface functionalization technique to chemically enhance live microbial cells.Molecular systems biology · 2026Article
- Controlling Cell Interactions with DNA Directed Assembly.Advanced healthcare materials · 2024Review
- Programming the Self-Organization of Endothelial Cells into Perfusable Microvasculature.Tissue engineering. Part A · 2023Article
- Harnessing synthetic biology to engineer organoids and tissues.Cell stem cell · 2023Review
- Detecting Intact Virus Using Exogenous Oligonucleotide Labels.Analytical chemistry · 2022Article
- DNA-Directed Patterning for Versatile Validation and Characterization of a Lipid-Based Nanoparticle Model of SARS-CoV-2.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Multiplexed DNA-Directed Patterning of Antibodies for Applications in Cell Subpopulation Analysis.ACS applied materials & interfaces · 2021Article
- Simple, Affordable, and Modular Patterning of Cells using DNA.Journal of visualized experiments : JoVE · 2021Article
- A quantitative assessment of the dynamic modification of lipid-DNA probes on live cell membranes.Chemical science · 2019Article
- Design Principles for Pluripotent Stem Cell-Derived Organoid Engineering.Stem cells international · 2019Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Tissues are the organizational units of function in metazoan organisms. Tissues comprise an assortment of cellular building blocks, soluble factors, and extracellular matrix (ECM) composed into specific three-dimensional (3-D) structures. The capacity to reconstitute tissues in vitro with the structural complexity observed in vivo is key to understanding processes such as morphogenesis, homeostasis, and disease. In this article, we describe DNA-programmed assembly of cells (DPAC), a method to fabricate viable, functional arrays of organoid-like tissues within 3-D ECM gels. In DPAC, dissociated cells are chemically functionalized with degradable oligonucleotide "Velcro," allowing rapid, specific, and reversible cell adhesion to a two-dimensional (2-D) template patterned with complementary DNA. An iterative assembly process builds up organoids, layer-by-layer, from this initial 2-D template and into the third dimension. Cleavage of the DNA releases the completed array of tissues that are captured and fully embedded in ECM gels for culture and observation. DPAC controls the size, shape, composition, and spatial heterogeneity of organoids and permits positioning of constituent cells with single-cell resolution even within cultures several centimeters long. © 2016 by John Wiley & Sons, Inc.
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