ArticlePloS one2016
Genome-Wide Assessment of Efficiency and Specificity in CRISPR/Cas9 Mediated Multiple Site Targeting in Arabidopsis.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed.
- Dynamic auxin maxima regulate male-to-hermaphrodite conversion and de novo meristem formation in the fern Ceratopteris gametophytes.PLoS biology · 2026Article
- Auxin affects gene editing efficiency through regulating chromatin accessibility and plant regeneration process.Horticulture research · 2025Article
- Turbo-charging crop improvement: harnessing multiplex editing for polygenic trait engineering and beyond.The Plant journal : for cell and molecular biology · 2025Review
- Applying conventional and cell-type-specific CRISPR/Cas9 genome editing in legume plants.aBIOTECH · 2025Review
- Chromosomal structural variation loci HSS1 and HSS6 lead to hybrid sterility in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Article
- Genome editing in plants using the TnpB transposase system.aBIOTECH · 2024Article
- Harnessing the potential of mutation breeding, CRISPR genome editing, and beyond for sustainable agriculture.Functional & integrative genomics · 2024Review
- In Silico Design of gRNA for CRISPR/Cas9-Mediated Gene Knockout.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Genome-wide evaluation of gene editing outcomes using CRISPR/Cas9 in seed propagatedFrontiers in plant science · 2024Article
- Mutational analysis of mechanosensitive ion channels in the carnivorous Venus flytrap plant.Current biology : CB · 2023Article
- A network of CLAVATA receptors buffers auxin-dependent meristem maintenance.Nature plants · 2023Article
- Recent Developments in CRISPR/Cas9 Genome-Editing Technology Related to Plant Disease Resistance and Abiotic Stress Tolerance.Biology · 2023Review
- Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance.Journal of Zhejiang University. Science. B · 2023Review
- Four class A AUXIN RESPONSE FACTORs promote tomato fruit growth despite suppressing fruit set.Nature plants · 2023Article
- Development of new mutant alleles and markers forFrontiers in plant science · 2023Article
- Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants.Frontiers in plant science · 2023Review
- SAUR63 stimulates cell growth at the plasma membrane.PLoS genetics · 2022Article
- Genome-wide specificity of plant genome editing by both CRISPR-Cas9 and TALEN.Scientific reports · 2022Article
- Function Analysis of theInternational journal of molecular sciences · 2022Article
24 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Simultaneous multiplex mutation of large gene families using Cas9 has the potential to revolutionize agriculture and plant sciences. The targeting of multiple genomic sites at once raises concerns about the efficiency and specificity in targeting. The model Arabidopsis thaliana is widely used in basic plant research. Previous work has suggested that the Cas9 off-target rate in Arabidopsis is undetectable. Here we use deep sequencing on pooled plants simultaneously targeting 14 distinct genomic loci to demonstrate that multiplex targeting in Arabidopsis is highly specific to on-target sites with no detectable off-target events. In addition, chromosomal translocations are extremely rare. The high specificity of Cas9 in Arabidopsis makes this a reliable method for clean mutant generation with no need to enhance specificity or adopt alternate Cas9 variants.
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