ArticleFrontiers in microbiology2016
Characterization and Strain Improvement of a Hypercellulytic Variant, Trichoderma reesei SN1, by Genetic Engineering for Optimized Cellulase Production in Biomass Conversion Improvement.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 45 citations in OpenAlex.
- Synergistic Fermentation Effect of Cellulose-Degrading Complex Microflora and Enzymes on Oat Grass Straw.Applied biochemistry and biotechnology · 2026Article
- From the bench to the reactor: engineered filamentous fungi for biochemical and biomaterial production.Biotechnology for biofuels and bioproducts · 2025Review
- Genomic Diversity and Phenotypic Variation in Fungal Decomposers Involved in Bioremediation of Persistent Organic Pollutants.Journal of fungi (Basel, Switzerland) · 2023Article
- A novel sucrose-inducible expression system and its application for production of biomass-degrading enzymes in Aspergillus niger.Biotechnology for biofuels and bioproducts · 2023Article
- The ERAD Pathway Participates in Fungal Growth and Cellulase Secretion inJournal of fungi (Basel, Switzerland) · 2023Article
- Development of a powerful synthetic hybrid promoter to improve the cellulase system of Trichoderma reesei for efficient saccharification of corncob residues.Microbial cell factories · 2022Article
- Novel Synergistic Mechanism for Lignocellulose Degradation by a Thermophilic Filamentous Fungus and a Thermophilic Actinobacterium Based on Functional Proteomics.Frontiers in microbiology · 2020Article
- The GATA-Type Transcriptional Factor Are1 Modulates the Expression of Extracellular Proteases and Cellulases inInternational journal of molecular sciences · 2019Article
- The influence of feedstock characteristics on enzyme production inBiotechnology for biofuels · 2019Review
- Production of highly efficient cellulase mixtures by genetically exploiting the potentials of Trichoderma reesei endogenous cellulases for hydrolysis of corncob residues.Microbial cell factories · 2017Article
- Development of a genome-editing CRISPR/Cas9 system in thermophilic fungalBiotechnology for biofuels · 2017Article
- Production of the versatile cellulase for cellulose bioconversion and cellulase inducer synthesis by genetic improvement ofBiotechnology for biofuels · 2017Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The filamentous fungus Trichoderma reesei is a widely used strain for cellulolytic enzyme production. A hypercellulolytic T. reesei variant SN1 was identified in this study and found to be different from the well-known cellulase producers QM9414 and RUT-C30. The cellulose-degrading enzymes of T. reesei SN1 show higher endoglucanase (EG) activity but lower β-glucosidase (BGL) activity than those of the others. A uracil auxotroph strain, SP4, was constructed by pyr4 deletion in SN1 to improve transformation efficiency. The BGL1-encoding gene bgl1 under the control of a modified cbh1 promoter was overexpressed in SP4. A transformant, SPB2, with four additional copies of bgl1 exhibited a 17.1-fold increase in BGL activity and a 30.0% increase in filter paper activity. Saccharification of corncob residues with crude enzyme showed that the glucose yield of SPB2 is 65.0% higher than that of SP4. These results reveal the feasibility of strain improvement through the development of an efficient genetic transformation platform to construct a balanced cellulase system for biomass conversion.
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