ArticleFrontiers in physiology2016
Validation of a Ramp Running Protocol for Determination of the True VO2max in Mice.
Article in Frontiers in physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Purinergic P2X7 Receptor Antagonism Impairs Aerobic Capacity without Detectable Effect on Cardiac Adaptation to Exercise in Mice.ACS pharmacology & translational science · 2026Article
- Sex differences in voluntary running behavior between C57BL/6 and BALB/cJ mouse strains do not correspond to changes in VO2 and RER.Physiological reports · 2025Article
- Establishing VO2peak-based exercise intensity classification and physiological validation in ICR mice.Physical activity and nutrition · 2025Article
- Animal Models of Exercise and Cardiometabolic Disease.Circulation research · 2025Review
- Myonuclear alterations associated with exercise are independent of age in humans.The Journal of physiology · 2025Article
- Critical Velocity, Maximal Lactate Steady State, and Muscle MCT1 and MCT4 after Exhaustive Running in Mice.International journal of molecular sciences · 2023Article
- Non-invasive assessment of HFpEF in mouse models: current gaps and future directions.BMC medicine · 2022Article
- Fine-Tuning Cardiac Insulin-Like Growth Factor 1 Receptor Signaling to Promote Health and Longevity.Circulation · 2022Article
- Loss of autophagy protein ATG5 impairs cardiac capacity in mice and humans through diminishing mitochondrial abundance and disrupting Ca2+ cycling.Cardiovascular research · 2022Article
- Physical exercise shapes the mouse brain epigenome.Molecular metabolism · 2021Article
- Deletion of CD73 increases exercise power in mice.Purinergic signalling · 2021Article
- Vitamin D supplementation associated with physical exercise promotes a tolerogenic immune environment without effect on mammary tumour growth in C57BL/6 mice.European journal of nutrition · 2021Article
- Systematic Review and Meta-Analysis of Endurance Exercise Training Protocols for Mice.Frontiers in physiology · 2021Review
- Neuronal adenosine AScientific reports · 2020Article
- Endothelial Lactate Controls Muscle Regeneration from Ischemia by Inducing M2-like Macrophage Polarization.Cell metabolism · 2020Article
- Guidelines for animal exercise and training protocols for cardiovascular studies.American journal of physiology. Heart and circulatory physiology · 2020Review
- The exercise sex gap and the impact of the estrous cycle on exercise performance in mice.Scientific reports · 2018Article
- Peak Velocity as an Alternative Method for Training Prescription in Mice.Frontiers in physiology · 2018Article
- Article
- Commentary: Validation of a Ramp Running Protocol for Determination of the True VOFrontiers in physiology · 2017Article
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5 authors.
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Abstract
In the field of comparative physiology, it remains to be established whether the concept of VO2max is valid in the mouse and, if so, how this value can be accurately determined. In humans, VO2max is generally considered to correspond to the plateau observed when VO2 no longer rises with an increase in workload. In contrast, the concept of VO2peak tends to be used in murine studies. The objectives of the present study were to determine whether (i) a continuous ramp protocol yielded a higher VO2peak than a stepwise, incremental protocol, and (ii) the VO2peak measured in the ramp protocol corresponded to VO2max. The three protocols (based on intensity-controlled treadmill running until exhaustion with eight female FVB/N mice) were performed in random order: (a) an incremental protocol that begins at 10 m.min(-1) speed and increases by 3 m.min(-1) every 3 min. (b) a ramp protocol with slow acceleration (3 m.min(-2)), and (c) a ramp protocol with fast acceleration (12 m.min(-2)). Each protocol was performed with two slopes (0 and 25°). Hence, each mouse performed six exercise tests. We found that the value of VO2peak was protocol-dependent (p < 0.05) and was highest (59.0 ml.kg (0.75).min(-1)) for the 3 m.min(-2) 0° ramp protocol. In the latter, the presence of a VO2max plateau was associated with the fulfillment of two secondary criteria (a blood lactate concentration >8 mmol.l(-1) and a respiratory exchange ratio >1). The total duration of the 3 m.min(-2) 0° ramp protocol was shorter than that of the incremental protocol. Taken as a whole, our results suggest that VO2max in the mouse is best determined by applying a ramp exercise protocol with slow acceleration and no treadmill slope.
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