ArticleProceedings of the National Academy of Sciences of the United States of America2016
Localizing gene regulation reveals a staggered wood decay mechanism for the brown rot fungus Postia placenta.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 187 citations in OpenAlex.
- Isolation, Identification ofInternational journal of molecular sciences · 2026Article
- Plant Biomass Depolymerization by Oxidoreductases: Structural Characteristics, Synergy Mechanism, and Evolutionary Adaptation.Journal of agricultural and food chemistry · 2026Review
- Reintroduction of lignin-modifying enzymes in the brown-rot fungus Gloeophyllum trabeum restores ligninolytic activities but diminishes lignocellulose degradation.Fungal biology and biotechnology · 2026Article
- Repeated measures of decaying wood reveal the success and influence of fungal wood endophytes.mSystems · 2025Article
- Secondary Decomposers Meet Their Predators: Decomposition Stage and Substrate Quality Jointly Structure Microbial Brown Food Webs During Fungal Necromass Decay.Molecular ecology · 2025Article
- Wood decay under anoxia by the brown-rot fungus Fomitopsis pinicola.Nature communications · 2025Article
- Two-stage wood decay byMycoscience · 2025Article
- Gene Expression by a Model Fungus in the Ascomycota Provides Insight Into the Decay of Fungal Necromass.Environmental microbiology · 2024Article
- Unlocking the distinctive enzymatic functions of the early plant biomass deconstructive genes in a brown rot fungus by cell-free protein expression.Applied and environmental microbiology · 2024Article
- Methionine oxidation of carbohydrate-active enzymes during white-rot wood decay.Applied and environmental microbiology · 2024Article
- Altered Expression of Two Small Secreted Proteins (International journal of molecular sciences · 2023Article
- Transcriptomics of Temporal- versus Substrate-Specific Wood Decay in the Brown-Rot FungusJournal of fungi (Basel, Switzerland) · 2023Article
- Applying molecular and genetic methods to trees and their fungal communities.Applied microbiology and biotechnology · 2023Review
- Extracellular enzymes secreted in the mycelial block of Lentinula edodes during hyphal growth.AMB Express · 2023Article
- A Laccase Gene Reporting System That Enables Genetic Manipulations in a Brown Rot Wood Decomposer FungusMicrobiology spectrum · 2023Article
- Current controversies on mechanisms controlling soil carbon storage: implications for interactions with practitioners and policy-makers. A review.Agronomy for sustainable development · 2023Review
- Comparative analysis of simulatedFrontiers in microbiology · 2023Article
- Metabolomics Highlights Different Life History Strategies of White and Brown Rot Wood-Degrading Fungi.mSphere · 2022Article
- Lignocellulose dissociation with biological pretreatment towards the biochemical platform: A review.Materials today. Bio · 2022Review
- X-Ray Scattering Reveals Two Mechanisms of Cellulose Microfibril Degradation by Filamentous Fungi.Applied and environmental microbiology · 2022Article
10 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wood-degrading brown rot fungi are essential recyclers of plant biomass in forest ecosystems. Their efficient cellulolytic systems, which have potential biotechnological applications, apparently depend on a combination of two mechanisms: lignocellulose oxidation (LOX) by reactive oxygen species (ROS) and polysaccharide hydrolysis by a limited set of glycoside hydrolases (GHs). Given that ROS are strongly oxidizing and nonselective, these two steps are likely segregated. A common hypothesis has been that brown rot fungi use a concentration gradient of chelated metal ions to confine ROS generation inside wood cell walls before enzymes can infiltrate. We examined an alternative: that LOX components involved in ROS production are differentially expressed by brown rot fungi ahead of GH components. We used spatial mapping to resolve a temporal sequence in Postia placenta, sectioning thin wood wafers colonized directionally. Among sections, we measured gene expression by whole-transcriptome shotgun sequencing (RNA-seq) and assayed relevant enzyme activities. We found a marked pattern of LOX up-regulation in a narrow (5-mm, 48-h) zone at the hyphal front, which included many genes likely involved in ROS generation. Up-regulation of GH5 endoglucanases and many other GHs clearly occurred later, behind the hyphal front, with the notable exceptions of two likely expansins and a GH28 pectinase. Our results support a staggered mechanism for brown rot that is controlled by differential expression rather than microenvironmental gradients. This mechanism likely results in an oxidative pretreatment of lignocellulose, possibly facilitated by expansin- and pectinase-assisted cell wall swelling, before cellulases and hemicellulases are deployed for polysaccharide depolymerization.
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