ArticleProceedings of the National Academy of Sciences of the United States of America2016
Toward combined delignification and saccharification of wheat straw by a laccase-containing designer cellulosome.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 101 citations in OpenAlex.
- Biological Nanoscaffolds from Hierarchical Construction to Applications.Molecules (Basel, Switzerland) · 2026Review
- Bio-inspired designer cellulosomes show strongest synergy on industrial substrates under natural-like conditions.Journal of industrial microbiology & biotechnology · 2026Article
- Creating a large designer cellulosome in yeast to boost ethanol production.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Structural and functional insights into cellulosomes: masters of plant cell wall degradation.Frontiers in microbiology · 2025Review
- Microbial mechanisms for higher hydrogen production in anaerobic digestion at constant temperature versus gradient heating.Microbiome · 2024Article
- A cellulosomal yeast reaction system of lignin-degrading enzymes for cellulosic ethanol fermentation.Biotechnology letters · 2024Article
- Improvement of Cellulomonas fimi endoglucanase CenA by multienzymatic display on a decameric structural scaffold.Applied microbiology and biotechnology · 2023Article
- Forages and Pastures Symposium: revisiting mechanisms, methods, and models for altering forage cell wall utilization for ruminants.Journal of animal science · 2023Article
- Carbohydrate Depolymerization by Intricate Cellulosomal Systems.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Designed High-Redox Potential Laccases Exhibit High Functional Diversity.ACS catalysis · 2022Article
- Low-abundance populations distinguish microbiome performance in plant cell wall deconstruction.Microbiome · 2022Article
- Conversion of the free Cellvibrio japonicus xyloglucan degradation system to the cellulosomal mode.Applied microbiology and biotechnology · 2022Article
- Evaluating Enzymatic Productivity-The Missing Link to Enzyme Utility.International journal of molecular sciences · 2022Review
- Combinatorial assembly and optimisation of designer cellulosomes: a galactomannan case study.Biotechnology for biofuels and bioproducts · 2022Article
- Stable and Functionally Diverse Versatile Peroxidases Designed Directly from Sequences.Journal of the American Chemical Society · 2022Article
- Biochemical characterization of a novel acidophilic β-xylanase fromFrontiers in microbiology · 2022Article
- Integrated engineering of enzymes and microorganisms for improving the efficiency of industrial lignocellulose deconstruction.Engineering microbiology · 2021Review
- An Engineered Thermostable Laccase with Great Ability to Decolorize and Detoxify Malachite Green.International journal of molecular sciences · 2021Article
- Wheat straw: A natural remedy against different maladies.Food science & nutrition · 2021Review
- Bioprospecting of microbial strains for biofuel production: metabolic engineering, applications, and challenges.Biotechnology for biofuels · 2021Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efficient breakdown of lignocellulose polymers into simple molecules is a key technological bottleneck limiting the production of plant-derived biofuels and chemicals. In nature, plant biomass degradation is achieved by the action of a wide range of microbial enzymes. In aerobic microorganisms, these enzymes are secreted as discrete elements in contrast to certain anaerobic bacteria, where they are assembled into large multienzyme complexes termed cellulosomes. These complexes allow for very efficient hydrolysis of cellulose and hemicellulose due to the spatial proximity of synergistically acting enzymes and to the limited diffusion of the enzymes and their products. Recently, designer cellulosomes have been developed to incorporate foreign enzymatic activities in cellulosomes so as to enhance lignocellulose hydrolysis further. In this study, we complemented a cellulosome active on cellulose and hemicellulose by addition of an enzyme active on lignin. To do so, we designed a dockerin-fused variant of a recently characterized laccase from the aerobic bacterium Thermobifida fusca The resultant chimera exhibited activity levels similar to the wild-type enzyme and properly integrated into the designer cellulosome. The resulting complex yielded a twofold increase in the amount of reducing sugars released from wheat straw compared with the same system lacking the laccase. The unorthodox use of aerobic enzymes in designer cellulosome machinery effects simultaneous degradation of the three major components of the plant cell wall (cellulose, hemicellulose, and lignin), paving the way for more efficient lignocellulose conversion into soluble sugars en route to alternative fuels production.
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