ArticleScientific reports2016
Fluorescence imaging of chromosomal DNA using click chemistry.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- A double-staining automated flow cytometry method for real-time monitoring of bacteria in continuous bioreactors.NPJ systems biology and applications · 2026Article
- The fluoropyrimidine polymer CF10 synergizes with 5-ethynyl-2'-deoxyuridine by promoting telomere attrition and mitotic catastrophe.NAR molecular medicine · 2026Article
- OpenEMMU: A versatile, open-source EdU multiplexing methodology for studying DNA replication and cell cycle dynamics.iScience · 2025Article
- Chemical Reactions in Living Systems.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- An Advanced Detection System forActa histochemica et cytochemica · 2022Article
- Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Review
- Covalent labeling of nucleic acids.Chemical Society reviews · 2020Review
- Conformation of G-quadruplex Controlled by Click Reaction.Molecules (Basel, Switzerland) · 2020Article
- Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association.Nature communications · 2020Article
- Comparison of the genetic relationship between nine Cephalopod species based on cluster analysis of karyotype evolutionary distance.Comparative cytogenetics · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromosome visualization is essential for chromosome analysis and genetic diagnostics. Here, we developed a click chemistry approach for multicolor imaging of chromosomal DNA instead of the traditional dye method. We first demonstrated that the commercially available reagents allow for the multicolor staining of chromosomes. We then prepared two pro-fluorophore moieties that served as light-up reporters to stain chromosomal DNA based on click reaction and visualized the clear chromosomes in multicolor. We applied this strategy in fluorescence in situ hybridization (FISH) and identified, with high sensitivity and specificity, telomere DNA at the end of the chromosome. We further extended this approach to observe several basic stages of cell division. We found that the click reaction enables direct visualization of the chromosome behavior in cell division. These results suggest that the technique can be broadly used for imaging chromosomes and may serve as a new approach for chromosome analysis and genetic diagnostics.
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Registered trials
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