ArticleAntimicrobial agents and chemotherapy2016
Development of a Novel Collagen Wound Model To Simulate the Activity and Distribution of Antimicrobials in Soft Tissue during Diabetic Foot Infection.
Article in Antimicrobial agents and chemotherapy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 43 citations in OpenAlex.
- Advancing preclinical research with reconstructed in vitro skin models mimicking non-healing wounds.International journal of pharmaceutics: X · 2026Review
- Development of a tri-species wound model for studying fungal-bacterial interactions and antimicrobial therapies.Biofilm · 2025Article
- Simulated Media for Mimicking the Human Environment In Vitro.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2025Review
- Absence of Synergism between a Dual-AMP Biogel and Antibiotics Used as Therapeutic Agents for Diabetic Foot Infections.International journal of molecular sciences · 2023Article
- Preclinical study of diabetic foot ulcers: From pathogenesis to vivo/vitro models and clinical therapeutic transformation.International wound journal · 2023Review
- Development of an All-Marine 3D Printed Bioactive Hydrogel Dressing for Treatment of Hard-to-Heal Wounds.Polymers · 2023Article
- Larval Therapy and Larval Excretions/Secretions: A Potential Treatment for Biofilm in Chronic Wounds? A Systematic Review.Microorganisms · 2023Review
- Boosting Cosmeceutical Peptides: Coupling Imidazolium-Based Ionic Liquids to Pentapeptide-4 Originates New Leads with Antimicrobial and Collagenesis-Inducing Activities.Microbiology spectrum · 2022Article
- Translational challenges and opportunities in biofilm science: a BRIEF for the future.NPJ biofilms and microbiomes · 2022Review
- Analysis of risk factors for multidrug-resistant organisms in diabetic foot infection.BMC endocrine disorders · 2022Article
- Milieu matters: AnBiofilm · 2021Article
- Disclosure of a Promising Lead to Tackle Complicated Skin and Skin Structure Infections: Antimicrobial and Antibiofilm Actions of Peptide PP4-3.1.Pharmaceutics · 2021Article
- Efficacy of Topical Vancomycin- and Gentamicin-Loaded Calcium Sulfate Beads or Systemic Antibiotics in Eradicating Polymicrobial Biofilms Isolated from Diabetic Foot Infections within anAntimicrobial agents and chemotherapy · 2021Article
- Alternative Approaches for the Management of Diabetic Foot Ulcers.Frontiers in microbiology · 2021Review
- Pexiganan in Combination with Nisin to Control Polymicrobial Diabetic Foot Infections.Antibiotics (Basel, Switzerland) · 2020Article
- Homalomena pineodora essential oil nanoparticle inhibits diabetic wound pathogens.Scientific reports · 2020Article
- Article
- An in vitro collagen perfusion wound biofilm model; with applications for antimicrobial studies and microbial metabolomics.BMC microbiology · 2019Article
- Diabetic Foot Ulcers: Current Advances in Antimicrobial Therapies and Emerging Treatments.Antibiotics (Basel, Switzerland) · 2019Review
- Bioengineered Platforms for Chronic Wound Infection Studies: How Can We Make Them More Human-Relevant?Frontiers in bioengineering and biotechnology · 2019Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Diabetes has major implications for public health, with diabetic foot ulcers (DFUs) being responsible for significant morbidity and mortality. A key factor in the development of nonhealing ulcers is infection, which often leads to the development of biofilm, gangrene, and amputation. A novel approach to treating DFUs is the local release of antibiotics from calcium sulfate beads. We have developed a novel model system to study and compare the release and efficacy of antibiotics released locally, using collagen as a substrate for biofilm growth and incorporating serum to mimic the biochemical complexity of the wound environment. We found that our soft-tissue model supports the growth of a robust Pseudomonas aeruginosa biofilm, and that this was completely eradicated by the introduction of calcium sulfate beads loaded with tobramycin or gentamicin. The model also enabled us to measure the concentration of these antibiotics at different distances from the beads and in simulated wound fluid bathing the collagen matrix. We additionally found that a multidrug-resistant Staphylococcus aureus biofilm, nonsusceptible to antibiotics, nonetheless showed an almost 1-log drop in viable counts when exposed to calcium sulfate beads combined with antibiotics. Together, these data suggest that locally applied antibiotics combined with calcium sulfate provide surprising efficacy in diabetic foot infections and offer an effective alternative approach to infection management. Our study additionally establishes our new system as a biochemically and histologically relevant model that may be used to study the effectiveness of a range of therapies locally or systemically for infected DFUs.
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