ArticleScientific reports2016
Metabolic engineering of cottonseed oil biosynthesis pathway via RNA interference.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 49 citations in OpenAlex.
- Bridging the Divide: Integrating Cottonseed Oil Content with Agronomic Trait Improvement in Upland Cotton (Plants (Basel, Switzerland) · 2026Review
- A review of flavonoids at the crossroads of plant defense: integrating biotic and abiotic stress tolerance through AI- and CRISPR/Cas-guided metabolic reprogramming.Frontiers in plant science · 2026Review
- Transcriptome Analysis Reveals Metabolic Pathways and Key Genes Involved in Oleic Acid Formation of Sunflower (International journal of molecular sciences · 2025Article
- Regulation of Oil Biosynthesis and Genetic Improvement in Plants: Advances and Prospects.Genes · 2024Review
- Comparative Phenotypic and Transcriptomic Analyses Provide Novel Insights into the Molecular Mechanism of Seed Germination in Response to Low Temperature Stress in Alfalfa.International journal of molecular sciences · 2024Article
- Hub Genes in Stable QTLs Orchestrate the Accumulation of Cottonseed Oil in Upland Cotton via Catalyzing Key Steps of Lipid-Related Pathways.International journal of molecular sciences · 2023Article
- Genetic and Morpho-Physiological Differences among Transgenic and No-Transgenic Cotton Cultivars.Plants (Basel, Switzerland) · 2023Article
- Integrated lipidomic and transcriptomic analyses reveal the mechanism of lipid biosynthesis and accumulation during seed development in sesame.Frontiers in plant science · 2023Article
- 4D genetic networks reveal the genetic basis of metabolites and seed oil-related traits in 398 soybean RILs.Biotechnology for biofuels and bioproducts · 2022Article
- Conservation and Divergence of Phosphoenolpyruvate Carboxylase Gene Family in Cotton.Plants (Basel, Switzerland) · 2022Article
- An integrated omics analysis reveals the gene expression profiles of maize, castor bean, and rapeseed for seed oil biosynthesis.BMC plant biology · 2022Article
- Development of an efficient and precise adenine base editor (ABE) with expanded target range in allotetraploid cotton (Gossypium hirsutum).BMC biology · 2022Article
- Genetics, Breeding and Genetic Engineering to Improve Cottonseed Oil and Protein: A Review.Frontiers in plant science · 2022Review
- Characterization of a novel cotton MYB gene, GhMYB108-like responsive to abiotic stresses.Molecular biology reports · 2020Article
- Proteomic analysis reveals that sugar and fatty acid metabolisms play a central role in sterility of the male-sterile line 1355A of cotton.The Journal of biological chemistry · 2019Article
- An integrated omics analysis reveals molecular mechanisms that are associated with differences in seed oil content between Glycine max and Brassica napus.BMC plant biology · 2018Article
- Comprehensive metabolic and transcriptomic profiling of various tissues provide insights for saponin biosynthesis in the medicinally important Asparagus racemosus.Scientific reports · 2018Article
- Engineered Dwarf Male-Sterile Rice: A Promising Genetic Tool for Facilitating Recurrent Selection in Rice.Frontiers in plant science · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cottonseed oil is recognized as an important oil in food industry for its unique characters: low flavor reversion and the high level of antioxidants (VitaminE) as well as unsaturated fatty acid. However, the cottonseed oil content of cultivated cotton (Gossypium hirsutum) is only around 20%. In this study, we modified the accumulation of oils by the down-regulation of phosphoenolpyruvate carboxylase 1 (GhPEPC1) via RNA interference in transgenic cotton plants. The qRT-PCR and enzyme activity assay revealed that the transcription and expression of GhPEPC1 was dramatically down-regulated in transgenic lines. Consequently, the cottonseed oil content in several transgenic lines showed a significant (P < 0.01) increase (up to 16.7%) without obvious phenotypic changes under filed condition when compared to the control plants. In order to elucidate the molecular mechanism of GhPEPC1 in the regulation of seed oil content, we quantified the expression of the carbon metabolism related genes of transgenic GhPEPC1 RNAi lines by transcriptome analysis. This analysis revealed the decrease of GhPEPC1 expression led to the increase expression of triacylglycerol biosynthesis-related genes, which eventually contributed to the lipid biosynthesis in cotton. This result provides a valuable information for cottonseed oil biosynthesis pathway and shows the potential of creating high cottonseed oil germplasm by RNAi strategy for cotton breeding.
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