ArticleBiochimica et biophysica acta2016
Isolation of yeast complex IV in native lipid nanodiscs.
Article in Biochimica et biophysica acta, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- Review
- pH-tunable membrane-active polymers, NCMNP2a-Chemical science · 2023Article
- Cryo-EM structure and function of S. pombe complex IV with bound respiratory supercomplex factor.Communications chemistry · 2023Article
- Detergent-Free Isolation of Membrane Proteins and Strategies to Study Them in a Near-Native Membrane Environment.Biomolecules · 2022Review
- Conformational trapping of an ABC transporter in polymer lipid nanoparticles.The Biochemical journal · 2022Article
- Mechanisms of Formation, Structure, and Dynamics of Lipoprotein Discs Stabilized by Amphiphilic Copolymers: A Comprehensive Review.Nanomaterials (Basel, Switzerland) · 2022Review
- Methods of Measuring Mitochondrial Potassium Channels: A Critical Assessment.International journal of molecular sciences · 2022Review
- Detergent-free systems for structural studies of membrane proteins.Biochemical Society transactions · 2021Review
- Biological insights from SMA-extracted proteins.Biochemical Society transactions · 2021Review
- A Three-Dimensional Model of the Yeast Transmembrane Sensor Wsc1 Obtained by SMA-Based Detergent-Free Purification and Transmission Electron Microscopy.Journal of fungi (Basel, Switzerland) · 2021Article
- Changes in Membrane Protein Structural Biology.Biology · 2020Review
- Membrane Protein Structure Determination and Characterisation by Solution and Solid-State NMR.Biology · 2020Review
- CD81 extracted in SMALP nanodiscs comprises two distinct protein populations within a lipid environment enriched with negatively charged headgroups.Biochimica et biophysica acta. Biomembranes · 2020Article
- Stabilization of Human Multidrug Resistance Protein 4 (MRP4/ABCC4) Using Novel Solubilization Agents.SLAS discovery : advancing life sciences R & D · 2019Article
- Single-particle cryo-EM studies of transmembrane proteins in SMA copolymer nanodiscs.Chemistry and physics of lipids · 2019Review
- Phosphatidylethanolamine made in the inner mitochondrial membrane is essential for yeast cytochrome bcNature communications · 2019Article
- Analysis of SMALP co-extracted phospholipids shows distinct membrane environments for three classes of bacterial membrane protein.Scientific reports · 2019Article
- Membrane protein nanoparticles: the shape of things to come.Biochemical Society transactions · 2018Review
- Styrene maleic acid derivates to enhance the applications of bio-inspired polymer based lipid-nanodiscs.European polymer journal · 2018Article
- Extraction and liposome reconstitution of membrane proteins with their native lipids without the use of detergents.Scientific reports · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 4 countries.
Funding
Abstract
We used the amphipathic styrene maleic acid (SMA) co-polymer to extract cytochrome c oxidase (CytcO) in its native lipid environment from S. cerevisiae mitochondria. Native nanodiscs containing one CytcO per disc were purified using affinity chromatography. The longest cross-sections of the native nanodiscs were 11nm×14nm. Based on this size we estimated that each CytcO was surrounded by ~100 phospholipids. The native nanodiscs contained the same major phospholipids as those found in the mitochondrial inner membrane. Even though CytcO forms a supercomplex with cytochrome bc
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.