Evidence map›Paper›PMID 27619528›Full record

ArticleBiosensors & bioelectronics2017

A sensitive DNA capacitive biosensor using interdigitated electrodes.

Lei Wang, Milena Veselinovic, Lang Yang, Brian J Geiss, David S Dandy, Tom Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed, 1 pooled it
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 1 synthesis or guideline pooled it, 107 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Review
  6. Biosensors for the detection of flaviviruses: A review.Synthetic and systems biotechnology · 2025
    Review
  7. Article
  8. Review
  9. Article
  10. Article
  11. Capacitive immunosensor for COVID-19 diagnosis.Microelectronic engineering · 2023
    Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Point-of-care human milk testing for maternal secretor status.Analytical and bioanalytical chemistry · 2022
    Article
  17. Review
  18. Evolution of nucleic acids biosensors detection limit III.Analytical and bioanalytical chemistry · 2022
    Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Lei WangSchool of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA.
Milena VeselinovicDepartment of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO 80523, USA.
Lang YangDepartment of Electrical & Computer Engineering, Colorado State University, Fort Collins, CO 80523, USA.
Brian J GeissSchool of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA; Department of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO 80523, USA.
David S DandySchool of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA; Department of Chemical & Biological Engineering, Colorado State University, Fort Collins, CO 80523, USA.
Tom ChenSchool of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA; Department of Electrical & Computer Engineering, Colorado State University, Fort Collins, CO 80523, USA. Electronic address: chen@engr.colostate.edu.
Colorado State University · US

Funding

A High-Throughput Screen for Antiviral Inhibitors of the Alphavirus RNA Capping EnzymeR01AI114675 · NIAID · COLORADO STATE UNIVERSITY · PI GEISS, BRIAN · 2015 to 2017
$1.1M
NIAID NIH HHS R01 AI114675
6 · The paper itself

Abstract

This paper presents a label-free affinity-based capacitive biosensor using interdigitated electrodes. Using an optimized process of DNA probe preparation to minimize the effect of contaminants in commercial thiolated DNA probe, the electrode surface was functionalized with the 24-nucleotide DNA probes based on the West Nile virus sequence (Kunjin strain). The biosensor has the ability to detect complementary DNA fragments with a detection limit down to 20 DNA target molecules (1.5aM range), making it suitable for a practical point-of-care (POC) platform for low target count clinical applications without the need for amplification. The reproducibility of the biosensor detection was improved with efficient covalent immobilization of purified single-stranded DNA probe oligomers on cleaned gold microelectrodes. In addition to the low detection limit, the biosensor showed a dynamic range of detection from 1µL

Indexed as

Electric CapacitanceNucleic Acid HybridizationBase SequenceBiosensing TechniquesDNADNA ProbesDNA, Single-StrandedElectrochemical TechniquesElectrodesEquipment DesignGoldHumansImmobilized Nucleic AcidsLimit of DetectionPoint-of-Care SystemsReproducibility of ResultsDNADNA ProbesDNA, Single-StrandedGoldImmobilized Nucleic AcidsSulfhydryl CompoundsAffinity-based capacitive biosensorInterdigitated electrodesNucleic acid-based biosensorPathogen detectionPoint-of-care diagnosticsViral diagnostics

Identifiers

PMID27619528
PMCPMC5295646
OpenAlexW2508965612

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.