ArticleJournal of experimental botany2016
Genome-scale mRNA and small RNA transcriptomic insights into initiation of citrus apomixis.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 62 citations in OpenAlex.
- The role of microRNAs in responses to drought and heat stress in peanut (Arachis hypogaea).The plant genome · 2023Pooled it
- Basis of apomixis in flowering plants - state of the art and research perspectives.Plant reproduction · 2026Review
- Paradigm shift in apomixis research: Targeting mutants with elements of apomixis in sexual crops.Planta · 2026Review
- A 24-nt miR9560 modulates the transporter gene BrpHMA2 expression in Brassica parachinensis.The plant genome · 2025Article
- Apomixis: oh, what a tangled web we have!Planta · 2023Review
- Data mining of transcriptional biomarkers at different cotton fiber developmental stages.Functional & integrative genomics · 2022Article
- Analysis of circRNAs and circRNA-associated competing endogenous RNA networks in β-thalassemia.Scientific reports · 2022Article
- The emerging role of small RNAs in ovule development, a kind of magic.Plant reproduction · 2021Review
- Transcriptomes and DNA methylomes in apomictic cells delineate nucellar embryogenesis initiation in citrus.DNA research : an international journal for rapid publication of reports on genes and genomes · 2021Article
- Small RNA sequencing provides candidate miRNA-target pairs for revealing the mechanism of apomixis in Zanthoxylum bungeanum.BMC plant biology · 2021Article
- A study of the heterochronic sense/antisense RNA representation in florets of sexual and apomictic Paspalum notatum.BMC genomics · 2021Article
- Small Non-Coding RNAs at the Crossroads of Regulatory Pathways Controlling Somatic Embryogenesis in Seed Plants.Plants (Basel, Switzerland) · 2021Review
- ZbAGL11, a class D MADS-box transcription factor of Zanthoxylum bungeanum, is involved in sporophytic apomixis.Horticulture research · 2021Article
- Genome-wide sRNA and mRNA transcriptomic profiling insights into dynamic regulation of taproot thickening in radish (Raphanus sativus L.).BMC plant biology · 2020Article
- Genome-wide analysis of the citrus B3 superfamily and their association with somatic embryogenesis.BMC genomics · 2020Article
- Review
- Small RNA and mRNA Sequencing Reveal the Roles of microRNAs Involved in Pomegranate Female Sterility.International journal of molecular sciences · 2020Article
- LINC00459 sponging miR-218 to elevate DKK3 inhibits proliferation and invasion in melanoma.Scientific reports · 2019Article
- Article
- Small RNA-seq reveals novel regulatory components for apomixis in Paspalum notatum.BMC genomics · 2019Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucellar embryony (NE) is an adventitious form of apomixis common in citrus, wherein asexual embryos initiate directly from nucellar cells surrounding the embryo sac. NE enables the fixation of desirable agronomic traits and the production of clonal offspring of virus-free rootstock, but impedes progress in hybrid breeding. In spite of the great importance of NE in citrus breeding and commercial production, little is understood about the underlying molecular mechanisms. In this study, the stages of nucellar embryo initiation (NEI) were determined for two polyembryonic citrus cultivars via histological observation. To explore the genes and regulatory pathways involved in NEI, we performed mRNA-seq and sRNA-seq analyses of ovules immediately prior to and at stages during NEI in the two pairs of cultivars. A total of 305 differentially expressed genes (DEGs) were identified between the poly- and monoembryonic ovules. Gene ontology (GO) analysis revealed that several processes are significantly enriched based on DEGs. In particular, response to stress, and especially response to oxidative stress, was over-represented in polyembryonic ovules. Nearly 150 miRNAs, comprising ~90 conserved and ~60 novel miRNAs, were identified in the ovules of either cultivar pair. Only two differentially expressed miRNAs (DEMs) were identified, of which the novel miRN23-5p was repressed whereas the targets accumulated in the polyembryonic ovules. This integrated study on the transcriptional and post-transcriptional regulatory profiles between poly- and monoembryonic citrus ovules provides new insights into the mechanism of NE, which should contribute to revealing the regulatory mechanisms of plant apomixis.
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